Abstract
In this study, we intend to explore the potential function of l-ascorbic acid in hypoxia-reoxygenation (H/R)-induced damage of CMECs and its related molecular mechanism. With different concentrations of l-ascorbic acid treatment, the proliferation, migration, inflammation and autophagy of cardiac microvascular endothelial cells (CMECs) were determined by several biological experiments. Si-HMGB1 transfection was used to reduce HMGB1 expression and to detect the function of HMGB1 in H/R-induced damage of CMECs. Under H/R condition, the proliferation and migration abilities of CMECs were reduced, and the inflammation and autophagy of CMECs were increased. Whereas, after l-ascorbic acid treatment, the reduction in the proliferation and migration of CMECs, as well as the increase in the inflammation and autophagy of CMECs induced by H/R were reversely altered. HMGB1 was confirmed as a specific target of l-ascorbic acid, and si-HMGB1 treatment strengthened the beneficial effect of l-ascorbic acid on H/R-induced damage of CMECs, followed by further reduction in the proliferation and migration abilities of CMECs, as well as the increase in the inflammation and autophagy of CMECs. Few studies have reported the function of l-ascorbic acid in myocardial ischemia on CMECs, but our experimental data showed that l-ascorbic acid treatment could ameliorate the H/R-induced damage of CMECs by regulating HMGB1 expression.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Our research was supported by Science and Technology Project of Zhangjiakou city (1621091D).
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Zhanshuai Zhang designed and performed the experiments and wrote the manuscript. Shaoqiang Qin conceived, supervised the experiments and wrote the manuscript. Yaling Wang performed the experiments and wrote the manuscript. Huiqing Liang analyzed the data and wrote the manuscript. Rui Wang analyzed the experiments and wrote the manuscript. Fangjiang Li analyzed the data and wrote the manuscript. All authors read and approved the final version.
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Zhang, Z., Qin, S., Wang, Y. et al. L-ascorbic acid could ameliorate the damage of myocardial microvascular endothelial cell caused by hypoxia-reoxygenation via targeting HMGB1. J Bioenerg Biomembr 55, 115–122 (2023). https://doi.org/10.1007/s10863-023-09962-x
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DOI: https://doi.org/10.1007/s10863-023-09962-x