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Evaluating the Effect of pH, Temperature and Concentration on Antioxidant and Antibacterial Potential of Spectroscopically, Spectrophotometrically and Microscopically Characterized Mentha Spicata Capped Silver Nanoparticles

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Abstract

The use of traditional plants has been tremendously increased due to their higher biological impact, minimal side effects, and comparatively low cost. Moreover, the emergence of antibacterial resistance is also shifting the scientific community to reconsider herbal remedies which provide relatively safer, cheap and biologically tolerable solutions. The present research was designed to fabricate the Mentha spicata conjugated silver nanoparticles (Me-AgNPs). Furthermore, the assessment of the bactericidal potential of Me-AgNPs against various bacterial strains was another motive behind this study. Fabricated NPs were characterized with the help of the UV-Visible spectrophotometric analysis, Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). Me-AgNPs showed a significant zone of inhibition (23 ± 0.2 mm) at 8 mg/mL against Staphylococcus aureus and a 4.0 ± 0.2 mm zone of growth inhibition at 2 mg/mL against Aeromonas veronii. The stability of Me-AgNPs was assessed at various pH (4, 7 and 11) and temperatures (25 °C, 4 °C, 37 °C, 75 °C). The significant zones of inhibition (11.3 ± 0.3 mm, 8.3 ± 0.3mm, 14.3 ± 0.3 mm, and 7.6 ± 0.2 mm) were observed at pH 11 against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae, respectively. Growth inhibition zones (14.0 ± 0.5 mm and 13.0 ± 0.5 mm) were also determined against B. subtilis and S. aureus at 25 °C. DPPH bioassay was conducted to find the antioxidant properties of Me-AgNPs. The highest (38.66 ± 0.2%) free radical scavenging activity was shown by Me-AgNPs at 4 mg/mL. Present study results concluded that biogenic Me-AgNPs have bactericidal as well as anti-oxidative potential. Moreover, these green synthesized Me-AgNPs could maintain their potency and stability at a wide range of pH and temperature.

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Funding

Current study was not funded by anybody/organization.

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Authors and Affiliations

  1. Applied Entomology and Medical Toxicology Laboratory, Department of Zoology, Government College University, 54000, Lahore, Pakistan

    Tahreem Tanveer, Shaukat Ali, Nazish Mazhar Ali, Muhammad Adeel Farooq, Muhammad Summer, Ali Hassan, Fareha Ali, Hafsa Shahzad & Rahila Islam

  2. Department of Biotechnology, University of Sargodha, Sargodha, Pakistan

    Muhammad Irfan

  3. Department of Zoology, University of Okara, Okara, Pakistan

    Lubna Kanwal

Authors
  1. Tahreem Tanveer
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  2. Shaukat Ali
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  6. Ali Hassan
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Contributions

T. T. performed the experiment and wrote the manuscript. S.A. supervised the experiment and edited the manuscript. N. M. A. supervised the experiment and edited the manuscript. M.A.F. edited the manuscript. M.S. edited the manuscript and characterization analysis. A. H. assisted in writeup and reference management. F. A. performed the experiment and wrote the manuscript. M. I. edited the manuscript. L. K. edited the manuscript. H. S. performed the experiment. R. I. managed the references.

Corresponding author

Correspondence to Shaukat Ali.

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As no animal is being used/sacrificed in present study, ethical approval was not applicable.

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Research Highlights

• Novel, eco-friendly and cost-effective Mentha spicata-conjugated silver nanoparticles were synthesized.

• Fabricated NPs were characterized with the help of the UV-Visible spectrophotometric analysis, Fourier transform infrared spectroscopy and Scanning electron microscopy.

• The stability of Mentha spicata-conjugated silver nanoparticles synthesized at various temperature and pH values was analyzed.

• The Mentha spicata conjugated silver nanoparticles were found effective against various bacterial pathogens.

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Tanveer, T., Ali, S., Ali, N.M. et al. Evaluating the Effect of pH, Temperature and Concentration on Antioxidant and Antibacterial Potential of Spectroscopically, Spectrophotometrically and Microscopically Characterized Mentha Spicata Capped Silver Nanoparticles. J Fluoresc 34, 1253–1267 (2024). https://doi.org/10.1007/s10895-023-03322-z

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