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Antimicrobial activity of green silver nanoparticles produced using aqueous leaf extract of Hydrocotyle rotundifolia

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Abstract

In the present work, colloidal silver nanoparticles (AgNPs) were synthesized in a single step process by green biosynthetic method using aqueous leaf extracts of an abundantly available medicinal plant Hydrocotyle rotundifolia. The AgNPs synthesized were characterized by UV-Vis spectroscopy, Transmission Electron Microscopy (TEM), Energy-Dispersive X-ray (EDX), Selected Area Electron Diffraction (SAED) and Fourier transform infrared (FTIR) spectroscopy. The UV-Vis absorption spectra of colloidal silver nanoparticles showed characteristic surface plasmon resonance (SPR) peak centred at 410 nm. The high resolution TEM images showed the as-synthesized nanoparticles are mostly spherical with and some elliptical in shape having average diameter of about 7.39 nm. FTIR analysis clearly revealed that the biomolecules present in the leaf extract of H. rotundifolia could be responsible for reduction of silver ion as well as prolonged stability of nanoparticles. The as-synthesized nanocolloids were stable and no precipitation was observed up to 3-months incubation at room temperature. The synthesized AgNPs were tested for its antimicrobial property against E. coli (DH5α). The MIC value was recorded as 5 μg/mL and demonstrated significant growth inhibition on agar plate. This eco-friendly and economical method may be a good option for the large scale preparation of silver nanoparticles for application in pharmaceutical, food, textiles and cosmetic industries.

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Acknowledgments

The corresponding author is sincerely thanks to Prof. Uday Shanker Dixit, Ex-Director, Central Institute of Technology, Kokrajhar for encouragement and his continuous support during the course of research work. We also like to acknowledge Department of Biotechnology, Haldia Institute of Technology for laboratory support during analysis of microbial experiments.

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Authors and Affiliations

  1. Department of Food Engineering and Technology, Central Institute of Technology, BTAD, Kokrajhar, Assam, 783370, India

    Ragini Kumari, Girija Brahma, Sumit Rajak & Santosh Kumar

  2. Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India

    Mukesh Singh

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  1. Ragini Kumari
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  2. Girija Brahma
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  3. Sumit Rajak
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  4. Mukesh Singh
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  5. Santosh Kumar
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Correspondence to Mukesh Singh or Santosh Kumar.

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Kumari, R., Brahma, G., Rajak, S. et al. Antimicrobial activity of green silver nanoparticles produced using aqueous leaf extract of Hydrocotyle rotundifolia . Orient Pharm Exp Med 16, 195–201 (2016). https://doi.org/10.1007/s13596-016-0236-8

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  • DOI: https://doi.org/10.1007/s13596-016-0236-8

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