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Antibacterial Activity of CdTe/ZnS Quantum Dot-β Lactum Antibiotic Conjugates

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Abstract

β-Lactum antibiotics are broad class of antibiotics which kills bacteria by inhibiting the formation of peptidoglycan that constitutes the bacterial cell wall. The resistance that develops in bacteria for antibiotics led the scientific world to think about the future aspects for modifying the way through which antibiotics are acted on the bacteria and become lethal for them. In this consequence, the potential of latest marketed antibiotics e.g. Amoxiciline (I), ceftazidim (II) have been evaluated after being conjugated with quantum dots. The surface of quantum dots has been conjugated with antibiotics by carbodiimide coupling with the help of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as conjugating agent between antibiotic and functionalized quantum dots. The antibacterial properties of QD-conjugated antibiotics have been determined by disc diffusion assay. The potency of QD-conjugated antibiotics has been estimated by determining their MIC50 for the selected strain of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Minimum inhibitory concentration study, minimum bactericidal concentration and growth pattern analysis revealed that QD-antibiotic conjugates showed slightly more prospective than pure native antibiotics against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria.

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Acknowledgment

We are thankful to sophisticated analytical instrument research facility (AIRF) Jawaharlal Lal Nehru University (JNU), New Delhi for TEM analysis. We are also grateful to Prof. M.K. Deb, School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur for FTIR analysis. Authors are thankful to the head, School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur for providing laboratory facilities. Authors are thankful to the head, School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur for providing laboratory facilities.

Funding

Financial assistance from DST-FIST and SAP is acknowledged with appreciation.

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Authors and Affiliations

  1. School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, C.G., 492010, India

    Jyoti Korram & Manmohan L. Satnami

  2. State Forensic Science Laboratory, Police line Campus, Tikrapara, Raipur, C.G., 492001, India

    Sandeep K. Vaishanav

  3. School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C.G., 492010, India

    S. K. Jadhav

  4. Department of Chemistry, Govt. Madhav P. G. Science College, Ujjain, M. P., 456010, India

    Rekha Nagwanshi

  5. Laxman Prasad Baidh Govt. Girls College, Bemetara, C.G., 491335, India

    Tikendra K. Verma

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  1. Sandeep K. Vaishanav
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Contributions

S.K.V.: experiment, data interpretation and drafting of the manuscript; J.K.: some measurement of fluorescence; R.N.: correction and review of manuscript; T.V.: helped in performing the antibacterial activity experiment and interpretation; S.K.J.: supervise all the antibacterial activity experiment and interpretation; M.L.S.: supervise all the experiment, interpretation of data and manuscript writing.

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Correspondence to Manmohan L. Satnami.

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Vaishanav, S.K., Korram, J., Verma, T.K. et al. Antibacterial Activity of CdTe/ZnS Quantum Dot-β Lactum Antibiotic Conjugates. J Fluoresc 34, 833–846 (2024). https://doi.org/10.1007/s10895-023-03316-x

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