Abstract
A red-emitting fluorescent Riboflavin (RF)/Polyvinylpyrrolidone (PVP)-coated silver nanoparticles system, λem = 527 nm, Φ = 0.242, with a diameter of the metallic core of 27.33 nm and a zeta potential of − 25.05 mV was prepared and investigated regarding its biological activity. We found that PVP has a key role in RF adsorption around the SNPs surface leading to an enhancement of antioxidant properties (∼70%), low cytotoxicity (> 90% cell viability, at 50 µL/mL, after 48 h of incubation) as well as to an efficient process of its cellular uptake (∼ 60%, after 24 h of incubation) in L929 cells. The results are relevant concerning the involvement of RF and its coenzymes forms in SNPs - based systems, in cellular respiration as well as for future studies as antioxidant marker system on tumoral cells for viewing and monitoring them, by cellular imaging.
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Acknowledgements
This work was done within the research programme “Quantum Chemistry and Molecular Structure” of the Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy. The authors thank Dr. Ana-Maria Seciu-Grama, from the National Institute of R&D for Biological Sciences, Bucharest, Romania, for flow cytometry measurements.
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Conceptualization, M.V.; Methodology, M.V, O.C, M.A.,V.S.M., D.C.C; Experimental Data, M.V., R.T.; M.A., J.M. C-M, V.S.M., D.C.C; Data Analysis, M.V, O.C, L.M.; Writing – Original Draft Preparation, M.V.; Writing – Review & Editing, M.V., O.C.; Visualization, M.V.
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Voicescu, M., Craciunescu, O., Calderon-Moreno, J.M. et al. Fluorescent Flavin/PVP-Coated Silver Nanoparticles: Design and Biological Performance. J Fluoresc 32, 1309–1319 (2022). https://doi.org/10.1007/s10895-022-02909-2
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DOI: https://doi.org/10.1007/s10895-022-02909-2