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Influence of Fe(III) on the Fluorescence of Lysozyme: a Facile and Direct Method for Sensitive and Selective Sensing of Fe(III)

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Abstract

Lysozyme is widely used for the synthesis of nanomaterials (e.g., gold nanoparticle) to fluorescently sense metal ions. However, the effect of metal ions on the fluorescence of lysozyme is not studied yet. Herein, we have explored the interactions of lysozyme with different metal ions to develop a direct sensing platform for Fe(III). It has been observed that the fluorescence of lysozyme was slightly decreased in the presence of Cu(II), Hg(II), As(V), Co(II), Cd(II), Cr(II), Fe(II), Mn(II), Pb(II), and Zn(II), while a significant decrease in the lysozyme fluorescence was observed for Fe(III). The effect of thermal stability on the fluorescence quenching was also studied from 25 to 60 °C. In the present study, the lysozyme sensing probe was able to selectively and accurately detect 0.5–50 ppm of Fe(III) with a LOD of 0.1 ppm (1.8 µM) at 25 °C.

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Acknowledgements

This research acknowledges the support from the Department of Science and Technology, New Delhi, India in the form of an INSPIRE Faculty Award (DST/INSPIRE/04/2017/002953) and from the Science and Engineering Research Board (SERB), Government of India under the Early Career Research (ECR) award (File No. ECR/2018/000748).

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Author notes

  1. Kalyan Vaid and Nikita Sarawagi have contributed equally to this work.

Authors and Affiliations

  1. National Agri-Food Biotechnology Institute (NABI), S.A.S. Nagar, Punjab, 140306, India

    Vanish Kumar, Kalyan Vaid, Nikita Sarawagi & Jasmeen Dhiman

  2. Centre for Nanoscience and Nanotechnology, Panjab University, Chandigarh, 160014, India

    Kalyan Vaid

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  1. Vanish Kumar
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  2. Kalyan Vaid
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Correspondence to Vanish Kumar.

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Kumar, V., Vaid, K., Sarawagi, N. et al. Influence of Fe(III) on the Fluorescence of Lysozyme: a Facile and Direct Method for Sensitive and Selective Sensing of Fe(III). J Fluoresc 31, 1815–1821 (2021). https://doi.org/10.1007/s10895-021-02813-1

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