Journal of Fluorescence

, Volume 28, Issue 2, pp 663–670 | Cite as

Highly Selective Detection of Cr3 + Ion with Colorimetric & Fluorescent Response Via Chemodosimetric Approach in Aqueous Medium

  • Ganesan Punithakumari
  • Shu Pao Wu
  • Sivan Velmathi


So far, very few numbers of chemosensors for Cr3+ ion have been reported. However, the main drawback of reported receptors are the lack of selectivity and other trivalent cations such as Fe3+, Al3+ and anions like F and OAc frequently interfere with such assays. This paper present the synthesis, characterization & sensor studies of Schiff base containing naphthalene moiety which selectively detect Cr3+ ion by chemodosimetric approach. Using FT-IR, 1H NMR, 13C NMR and ESI mass spectroscopic techniques the probe was characterized. This receptor exhibit more selectivity and sensitivity towards Cr3+ than other divalent and trivalent cations like Mn2+, Zn2+, Co2+, Ni2+, Cd2+, Cu2+, Hg2+, Fe3+, and Al3+ ions. After the addition of chromium ion the receptor get change from yellow to colorless in aqueous medium. But no color change was observed on the addition of other metal ions. Using UV-Vis and PL studies, it was confirmed that the selective hydrolysis of imine group of receptor by Cr3+ ions takes place with high fluorescence enhancement that is corresponding to 1-naphthylamine. Receptor acts as selective chemodosimeter for Cr3+ ions with 2:1 stoichiometry and micro molar detection limit. This chemodosimetric approach was applied successfully for bio-imaging of HeLa cells.


Naphthalene Schiff base Cr(III) Turn-on fluorescence Bio-imaging Chemodosimeter 



Authors thank the MHRD, GOI for providing infra structure facilities.

Supplementary material

10895_2018_2228_MOESM1_ESM.docx (815 kb)
ESM 1 (DOCX 814 kb)


  1. 1.
    Johnson J (1998) Mercury summit planned. Chem Eng News 76:22–23CrossRefGoogle Scholar
  2. 2.
    Carapuca HM, Monterroso SCC, Rocha LS, Duarte AC (2004) Simultaneous determination of copper and lead in seawater using optimised thin-mercury film electrodes in situ plated in thiocyanate media. Talanta 64:566–569CrossRefPubMedGoogle Scholar
  3. 3.
    Honda K, Sahrul M, Hidaka H, Tatsakana R (1983) Organ and tissue distribution of heavy metals, and their growth-related changes in Antarctic fish. Agric Biol Chem 47:2521–2531Google Scholar
  4. 4.
    Kotas J, Stasicka Z (2000) Chromium occurrence in the environment and methods of its speciation. Environ Pollut 107:263–283CrossRefPubMedGoogle Scholar
  5. 5.
    Goswami S, Ghosh UC (2005) Studies on adsorption behaviour of Cr (VI) onto synthetic hydrous stannic oxide. Water SA 31:597–600Google Scholar
  6. 6.
    Deng G, Wu K, Cruce AA, Bowman MK, Vincent JB (2015) Binding of trivalent chromium to serum transferring is sufficiently rapid to be physiologically relevant. J Inorg Biochem 143:48–55CrossRefPubMedGoogle Scholar
  7. 7.
    Peng M, Yang XP (2015) Controlling diabetes by chromium complexes: the role of the ligands. J Inorg Biochem 146:97–103CrossRefPubMedGoogle Scholar
  8. 8.
    Codd R, Irwin JA, La PA (2003) Sialoglycoprotein and carbohydrate complexes in chromium toxicity. Curr Opin Chem Biol 7:213–219CrossRefPubMedGoogle Scholar
  9. 9.
    Di Bona KR, Love S, Rhodes NR, McAdory D, Halder Sinha S, Kent N, Kent J, Strickland J, Wilson A, Beaird J, Ramage J, Rasco JF, Vincent JB (2011) Chromium is not an essential trace element for mammals: effects of a “low-chromium” diet. J Biol Inorg Chem 16:381–390CrossRefPubMedGoogle Scholar
  10. 10.
    McRae R, Bagchi P, Sumalekshmy S, Fahrni CJ (2009) In situ imaging of metals in cells and tissues. Chem Rev 109:4780–4827CrossRefPubMedGoogle Scholar
  11. 11.
    Panda SK (2007) Chromium-mediated oxidative stress and ultrastructural changes in root cells of developing rice seedlings. J Plant Physiol 164:1419–1428CrossRefPubMedGoogle Scholar
  12. 12.
    Latva S, Jokiniemi J, Peraniemi S, Ahlgren M (2003) Separation of picogram quantities of Cr(III) and Cr(VI) species in aqueous solutions and determination by graphite furnace atomic absorption spectrometry. J Anal At Spectrom 18:84–86CrossRefGoogle Scholar
  13. 13.
    Kovacik J, Babula P, Klejdus B, Hedbavny J (2013) Chromium uptake and consequences for metabolism and oxidative stress in chamomile plants. J Agric Food Chem 61:7864–7873CrossRefPubMedGoogle Scholar
  14. 14.
    Brose DA, James BR (2010) Oxidation− reduction transformations of chromium in aerobic soils and the role of electron-shuttling quinones. Environ Sci Technol 44:9438–9444CrossRefPubMedGoogle Scholar
  15. 15.
    Zayed AM, Terry N (2003) Chromium in the environment: factors affecting biological remediation. Plant. Soil 249:139–156Google Scholar
  16. 16.
    Balan C, Donia B, Macoveanu M (2009) Studies on chromium (III) removal from aqueous solutions by sorption on sphagnum moss peat. J Serbian Soc 74:953–964CrossRefGoogle Scholar
  17. 17.
    Domaille DW, Que EL, Chang CJ (2008) Synthetic fluorescent sensors for studying the cell biology of metals. Nat Chem Biol 4:168–175CrossRefPubMedGoogle Scholar
  18. 18.
    Deng J, Yu P, Yang LL, Mao L (2013) Competitive coordination of Cu2+ between cysteine and pyrophosphate ion toward sensitive and selective sensing of pyrophosphate ion in synovial fluid of arthritis. Anal Chem 85:2516–2522CrossRefPubMedGoogle Scholar
  19. 19.
    Chen W, Li Q, Zheng W, Hu F, Zhang G, Wang Z, Zhang D, Jiang X (2014) Identification of bacteria in water by the fluorescent array. Angew 126:13954–13959CrossRefGoogle Scholar
  20. 20.
    Lim NC, Schuster JV, Porto MC, Tanudra MA, Yao L, Freake HC, Bruckner C (2005) A ratiometric and on–off fluorescent chemosensor for highly selective detection of Cr3+ ion based on an ICT mechanism. Inorg Chem 44:2018–2030CrossRefPubMedGoogle Scholar
  21. 21.
    Zhou Z, Yu M (2008) FRET-based sensor for imaging chromium (III) in living cells. Chem Commun 29:3387–3389CrossRefGoogle Scholar
  22. 22.
    Huang X, Fan C, Wang Z, Zhan X, Pei M, Lu Z (2015) Ratiometric and on-off fluorescent chemosensor for highly selective detection of Cr3+ ion based on an ICT mechanism. Inorg Chem Commun 57:62–65CrossRefGoogle Scholar
  23. 23.
    Gupta VK, Mergu N, Singh AK (2015) Rhodamine-derived highly sensitive and selective colorimetric and off-on optical chemosensors for Cr3+. Sens Actuator B- Chem 220:420–432CrossRefGoogle Scholar
  24. 24.
    Hu X, Chai J, Liu Y, Liu B, Yang B (2016) Chromium (III) from chromium(VI) in cells by a fluorescent sensor. Spectrochim. Acta Part A 153:505–509CrossRefGoogle Scholar
  25. 25.
    Sheng H, Meng X, Ye W, Feng Y, Sheng H (2014) A water-soluble fluorescent probe for Fe(III): improved selectivity over Cr(III). Sens. Actuator B- Chem 195:534–539CrossRefGoogle Scholar
  26. 26.
    Mao J, Wang L, Dou W, Tang X, Yan Y, Liu W (2007) Tuning the selectivity of two Chemosensors to Fe(III) and Cr(III). Org Lett 9:4567–4570CrossRefPubMedGoogle Scholar
  27. 27.
    Xie P, Guo F, Xiao Y, Jin Q, Yao D, Huang Z (2013) A fluorescent chemosensor based on rhodamine for Cr3+ in red spectral region in aqueous solutions and living cells. J Lumin 140:45–50CrossRefGoogle Scholar
  28. 28.
    Sunnapu O, Kotla NG, Maddiboyina B (2017) Rhodamine based effective chemosensor for chromium(III) and their application in live cell imaging. Sens. Actuator B-Chem 246:761–768CrossRefGoogle Scholar
  29. 29.
    Jung JY, Han SJ, Chun J, Lee C, Yoon J (2012) New thiazolothiazole derivatives as fluorescent chemosensors for Cr3+ and Al3+. Dyes Pigments 94:423–426CrossRefGoogle Scholar
  30. 30.
    Thale PB, Sahoo SK (2015) An “off–on” colorimetric chemosensor for selective detection of Al3+, Cr3+ and Fe3+: its application in molecular logic gate. Sens Actuator B-Chem 215:451–458CrossRefGoogle Scholar
  31. 31.
    Elavarasi M, Sruthi AA, Chandrasekaran N, Mukherjee A (2014) Simple fluorescence-based detection of Cr(III) and Cr(VI) using unmodified gold nanoparticles. Anal Methods 6:9554–9560CrossRefGoogle Scholar
  32. 32.
    Wang M, Wang J, Xue W, Wu A (2013) A benzimidazole-based ratiometric fluorescent sensor for Cr3+ and Fe3+ in aqueous solution. Dyes Pigments 97:475–480CrossRefGoogle Scholar
  33. 33.
    Bravo V, Gil S, Costero AM (2012) A new phenanthrene-based bis-oxime chemosensor for Fe(III) and Cr(III) discrimination. Tetrahedron Lett 68:4882–4887CrossRefGoogle Scholar
  34. 34.
    Janakipriya S, Chereddy NR (2016) Selective interactions of trivalent cations Fe3 +, Al3 + and Cr3+ turn on fluorescence in a naphthalimide based single molecular probe. Spectrochim Acta A Mol Biomol 153:465–470CrossRefGoogle Scholar
  35. 35.
    Samanta S, Goswami S, Ramesh A, Gopal Das A (2015) A new chemodosimetric probe for the selective detection of trivalent cations in aqueous medium and live cells. J of Photochem and Photobio A: Chem 310:45–51CrossRefGoogle Scholar
  36. 36.
    Jang YJ, Yeon YH, Yang HY, Noh JY, Hwang IH, Kim C (2013) A colorimetric and fluorescent chemosensor for selective detection of Cr3+ and Al3+. Inorg Chem Commun 33:48–51CrossRefGoogle Scholar
  37. 37.
    Weerasinghe AJ, Oyeamalu AN, Abebe FA (2016) Rhodamine based turn-on sensors for Ni2+ and Cr3+ in organic media: detecting CN via the metal displacement approach. J Fluoresc 26:891–898CrossRefPubMedGoogle Scholar
  38. 38.
    Yang Y, Xue H, Chen L (2013) Colorimetric and highly selective fluorescence “turn-on” detection of Cr3+ by using a simple Schiff Base sensor. Chin J Chem 31:377–380CrossRefGoogle Scholar
  39. 39.
    Erdemir S, Kocyigi O (2016) Anthracene Excimer-based “turn on” fluorescent sensor for Cr3+ and Fe3+ ions: its application to living cells. Talanta 156:63–69CrossRefGoogle Scholar
  40. 40.
    Guha S, Lohar S, Banerjee A, Sahana A (2012) Thiophene anchored coumarin derivative as a turn-on fluorescent probe for Cr3+: cell imaging and speciation studies. Talanta 91:18–25CrossRefPubMedGoogle Scholar
  41. 41.
    Du W, Shou W (2016) A 1, 8-naphthalimide-based chemosensor with an off-on fluorescence and lifetime imaging response for intracellular Cr3+ and further for S2−. Dyes Pigments 126:279–285CrossRefGoogle Scholar
  42. 42.
    Saluja P, Kaur N, Singh N (2012) Benzimidazole-based fluorescent sensors for Cr3+ and their resultant complexes for sensing HSO4 and F. Tetrahedron Lett 68:8551–8556CrossRefGoogle Scholar
  43. 43.
    Saluja P, Sharma H, Kaur N (2012) Benzimidazole-based imine-linked chemosensor: chromogenic sensor for Mg2+ and fluorescent sensor for Cr3+. Tetrahedron Lett 68:2289–2293CrossRefGoogle Scholar
  44. 44.
    Karak D, Banerjee A, Sahana A (2011) 9-Acridone-4-carboxylic acid as an efficient Cr(III) fluorescent sensor: trace level detection, estimation and speciation studies. J Hazard Mater 188:274–280CrossRefPubMedGoogle Scholar
  45. 45.
    Zhou Y, Zhang J, Zhang L, Zhang Q, Ma T, Niu J (2013) A rhodamine-based fluorescent enhancement chemosensor for the detection of Cr3+ in aqueous media. Dyes Pigments 97:148–154CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemistry, Organic and Polymer Synthesis LaboratoryNational Institute of TechnologyTiruchirapalliIndia
  2. 2.Department of Applied ChemistryNational Chiao Tung UniversityHsinchuTaiwan

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