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Study on the Interaction of the CpG Alternating DNA with CdTe Quantum Dots

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Abstract

A novel sensitive method for detection of DNA methylation was developed with thioglycollic acid (TGA)-capped CdTe quantum dots (QDs) as fluorescence probes. Recognition of methylated DNA sites would be useful strategy due to the important roles of methylation in disease occurrence and developmental processes. DNA methylation occurs most often at cytosine-guanine sites (CpG dinucleotides) of gene promoters. The QDs significantly interacted with hybridized unmethylated and methylated DNA. The interaction of CpG rich methylated and unmethylated DNA hybrid with quantum dots as an optical probe has been investigated by fluorescence spectroscopy and electrophoresis assay. The fluorescence intensity of QDs was highly dependent to unmethylated and methylated DNA. Specific site of CpG islands of Adenomatous polyposis coli (APC), a well-studied tumor suppressor gene, was used as the detection target. Under optimum conditions, upon the addition of unmethylated dsDNA, the fluorescence intensity increased in linear range from 1.0 × 10− 10 to 1.0 × 10− 6M with detection limit of 6.2 × 10− 11 M and on the other hand, the intensity of QDs showed no changes with addition of methylated dsDNA. We also demonstrated that the unmethylated and methylated DNA and QDs complexes showed different mobility in electrophoresis assay. This easy and reliable method could distinguish between methylated and unmethylated DNA sequences.

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Acknowledgements

The authors thank the research Council of University of Tehran for financial support of this work.

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Authors and Affiliations

  1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran

    Morteza Hosseini, Freshteh Khaki, Ehsan Shokri & Hossein Khabbaz

  2. Department of Biotechnology, Payame Noor University, Tehran, Iran

    Mehdi Dadmehr

  3. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran

    Mohammad Reza Ganjali

  4. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Ganjali

  5. School of Chemistry, Damghan University, Damghan, Iran

    Mina Feizabadi & Davood Ajloo

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  1. Morteza Hosseini
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  2. Freshteh Khaki
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  4. Hossein Khabbaz
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Correspondence to Morteza Hosseini.

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Hosseini, M., Khaki, F., Shokri, E. et al. Study on the Interaction of the CpG Alternating DNA with CdTe Quantum Dots. J Fluoresc 27, 2059–2068 (2017). https://doi.org/10.1007/s10895-017-2145-8

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  • DOI: https://doi.org/10.1007/s10895-017-2145-8

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