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Preparation of K+-Doped Core-Shell NaYF4:Yb, Er Upconversion Nanoparticles and its Application for Fluorescence Immunochromatographic Assay of Human Procalcitonin

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Abstract

In the present study, we reported a convenient route to prepare well dispersed and functionalized K+-doped core-shell upconversion nanoparticles (UCP) by layer-by-layer (LbL) assembly of polyelectrolytes. UCP was firstly transferred to aqueous phase using cationic surfactant cetyl trimethyl ammonium bromide (CTAB) via hydrophobic interaction without removing the existing oleic acid (OA). Then the positively charged hydrophilic UCP@CTAB was further alternately deposited with negatively charged [poly (sodium 4-styrenesulfonate)] (PSS), positively charged [poly (allylamine hydrochloride)] (PAH) and negatively charged [poly (acrylic acid)] (PAA). The final carboxyl functionalized UCP@CTAB@PSS@PAH@PAA was then conjugated with monoclonal antibody1 (AB1) of procalcitonin (PCT), resulting in successful detection of PCT antigens based on the immunochromatographic assay (ICA). Linear response was achieved from 0 to 10 ng/mL, and the lowest limit of detection (LLD) was 0.18 ng/mL.

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Acknowledgments

This study was founded by the National Natural Science Foundation of China (No.21176124). We thank to Nanjing Norman Biological Technology Co. Ltd. for providing the PCT antibodies.

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  1. College of Chemistry and Molecular Engineering, Nanjing Tech University, No.30 Puzhu Road(S), Nanjing, 211816, China

    Jie Tang, Lijiang Lei, Hui Feng, Hongman Zhang & Yuwang Han

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  1. Jie Tang
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Correspondence to Yuwang Han.

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Tang, J., Lei, L., Feng, H. et al. Preparation of K+-Doped Core-Shell NaYF4:Yb, Er Upconversion Nanoparticles and its Application for Fluorescence Immunochromatographic Assay of Human Procalcitonin. J Fluoresc 26, 2237–2246 (2016). https://doi.org/10.1007/s10895-016-1919-8

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