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A New Thiosemicarbazone-Based Fluorescence “Turn-on” Sensor for Zn2+ Recognition with a Large Stokes Shift and its Application in Live Cell Imaging

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Abstract

Selective fluorescence turn on Zn2+ sensor with long-wavelength emission and a large Stokes shift is highly desirable in Zn2+ sensing area. We reported herein the synthesis and Zn2+ recognition properties of a new thiosemicarbazone-based fluorescent sensor L. L displays high selectivity and sensitivity toward Zn2+ over other metal ions in DMSO-H2O (1:1, v/v, HEPES 10 mM, pH = 7.4) solution with a long-wavelength emission at 572 nm and a large Stokes shift of 222 nm. Confocal fluorescence microscopy experiments demonstrate that L is cell-permeable and capable of monitoring intracellular Zn2+.

We report a new thiosemicarbazone-based fluorescent sensor (L) for selective recognition of Zn2+ with a long wavelength emission and a large Stokes shift.

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Acknowledgments

This project was supported by National Natural Science Foundation of China (Grant Nos. 21176029, 21476029), Liaoning BaiQianWan Talents Program (No. 2012921057), and the Program for Liaoning Excellent Talents in University (No. LR2015001).

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Authors and Affiliations

  1. Department of Chemistry, Bohai University, Jinzhou, 121013, China

    Lijun Tang, Zhenlong Huang, Zhuxuan Zheng, Keli Zhong & Yanjiang Bian

  2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-tech District, Dalian, 116024, China

    Zhenlong Huang

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Correspondence to Lijun Tang.

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Tang, L., Huang, Z., Zheng, Z. et al. A New Thiosemicarbazone-Based Fluorescence “Turn-on” Sensor for Zn2+ Recognition with a Large Stokes Shift and its Application in Live Cell Imaging. J Fluoresc 26, 1535–1540 (2016). https://doi.org/10.1007/s10895-016-1827-y

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