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A New Thiophenyl Pyrazoline Fluorescent Probe for Cu2+ in Aqueous Solution and Imaging in Live Cell

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Abstract

A new thiophenyl pyrazoline probe for Cu2+ in aqueous solution was synthesized and characterized by IR, NMR, HRMS and X-ray analysis. The probe displays remarkably high selectivity and sensitivity for Cu2+ with a detection limit of 1.919 × 10−7 M in aqueous solution (EtOH:HEPES = 1:1, v/v, 0.02 M, pH = 7.2). In addition, the probe is further successfully used to image Cu2+ in living cells and the probe possesses good reversibility.

A new thiophenyl pyrazoline probe can be used to image Cu2+ in living cells with good reversibility

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Acknowledgments

This study was supported by 973 Program (2010CB933504) and National Natural Science Foundation of China (90813022 and 20972088).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People’s Republic of China

    Meng-Meng Li, Ting-Ting Zhang, Wei-Liu Fan, Yu Xu, Yu Xiao & Bao-Xiang Zhao

  2. School of Life Science, Shandong University, Jinan, 250100, People’s Republic of China

    Wen-Bo Zhao & Jun-Ying Miao

Authors
  1. Meng-Meng Li
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  2. Wen-Bo Zhao
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  3. Ting-Ting Zhang
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  4. Wei-Liu Fan
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  5. Yu Xu
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  6. Yu Xiao
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  7. Jun-Ying Miao
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  8. Bao-Xiang Zhao
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Corresponding authors

Correspondence to Jun-Ying Miao or Bao-Xiang Zhao.

Additional information

Meng-Meng Li and Wen-Bo Zhao contributed equally to this work.

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Li, MM., Zhao, WB., Zhang, TT. et al. A New Thiophenyl Pyrazoline Fluorescent Probe for Cu2+ in Aqueous Solution and Imaging in Live Cell. J Fluoresc 23, 1263–1269 (2013). https://doi.org/10.1007/s10895-013-1259-x

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  • DOI: https://doi.org/10.1007/s10895-013-1259-x

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