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Novel Fluorescent Membrane for Metronidazole Sensing Prepared by Covalent Immobilization of a Pyrenebutyric Acid Derivative

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Abstract

In the present paper, we report the fabrication of a new sensing membrane for fluorescence detection of metronidazole (MNZ). Briefly, a pyrenebutyric acid derivative, 2-(methacryloyloxy) ethyl-4-(1-pyrenyl) butanoate (MPB) with a double bond, was synthesized and copolymerized with 2-hydroxyethylmethacrylate (HEMA) on the activated glass surface by thermal initiation in the presence of cross-linker. The sensor responds linearly to metronidazole in the concentration range of 1.23~35.48 mg.L−1 in aqueous solution with a detection limit of 0.36 mg.L−1. The lifetime is enhanced by covalently immobilizing the pyrenebutyric acid derivative on glass slide, which hinders leaching of the dye from the membrane. The sensor could be regenerated after use by washing in methanol (RSD = 2.42 %), and it shows sufficient stability, and selectivity. Interference of other pharmaceuticals on membrane performance is discussed. The developed membrane has been successfully applied for the direct determination of metronidazole in human serum sample without pretreatment.

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Acknowledgments

The author would like to thank Dr. Jian Zhang at Department of Biochemistry and Molecular Biology, University of Maryland, for informative discussions. This work was supported by the National Natural Science Foundation of China (21065011, 20765005).

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Authors and Affiliations

  1. College of Chemistry & Chemical Engineering, Xinjiang University, Xinjiang Key laboratory of Oil and Gas Fine Chemicals, Urumqi, 830046, China

    Asigul Muhammad, Turghun Muhammad, Osmanjan Yimit & Burabiye Yakup

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  1. Asigul Muhammad
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  2. Turghun Muhammad
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  3. Osmanjan Yimit
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  4. Burabiye Yakup
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Correspondence to Turghun Muhammad.

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Muhammad, A., Muhammad, T., Yimit, O. et al. Novel Fluorescent Membrane for Metronidazole Sensing Prepared by Covalent Immobilization of a Pyrenebutyric Acid Derivative. J Fluoresc 23, 599–604 (2013). https://doi.org/10.1007/s10895-013-1176-z

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  • DOI: https://doi.org/10.1007/s10895-013-1176-z

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