Skip to main content
SpringerLink
Log in

Enzyme-responsive Fluorescent Ionic Liquid

  • Rapid Communications
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Herein, we describe the development of a novel material, “enzyme-responsive fluorescent ionic liquid”, which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALP- labeled antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. E. Bakker, P. Bühlmann, and E. Pretsch, Chem. Rev., 1997, 97, 3083.

    Article  CAS  PubMed  Google Scholar 

  2. P. Bühlmann, E. Pretsch, and E. Bakker, Chem. Rev., 1998, 98, 1593.

    Article  PubMed  Google Scholar 

  3. G. Mistlberger, G. A. Crespo, and E. Bakker, Annu. Rev. Anal. Chem., 2014, 7, 483.

    Article  Google Scholar 

  4. H. Hisamoto, Y. Nakashima, C. Kitamura, S. Funano, M. Yasuoka, K. Morishima, Y. Kikutani, T. Kitamori, and S. Terabe, Anal. Chem., 2004, 76, 3222.

    Article  CAS  PubMed  Google Scholar 

  5. H. Hisamoto, M. Yasuoka, and S. Terabe, Anal. Chim. Acta, 2006, 556, 164.

    Article  CAS  PubMed  Google Scholar 

  6. E. Tsutsumi, T. G. Henares, K. Kawamura, T. Yao, and H. Hisamoto, Chem. Lett., 2010, 39, 436.

    Article  CAS  Google Scholar 

  7. S. Funano, M. Sugahara, T. G. Henares, K. Sueyoshi, T. Endo, and H. Hisamoto, Analyst, 2015, 140, 1459.

    Article  CAS  PubMed  Google Scholar 

  8. L. C. Branco and F. Pina, Chem. Commun., 2009, 6204.

    Google Scholar 

  9. J. Avó, L. Cunha-Silva, J. C. Lima, and A. J. Parola, Org. Lett., 2014, 16, 2582.

    Article  PubMed  Google Scholar 

  10. S. Das, P. K. S. Magut, S. L. de Rooy, F. Hasan, and I. M. Warner, RSC Adv., 2013, 3, 21054.

    Article  CAS  PubMed  Google Scholar 

  11. H. Enozawa, S. Ukai, H. Ito, T. Murata, and Y. Morita, Org. Lett., 2019, 21, 2161.

    Article  CAS  PubMed  Google Scholar 

  12. K. Y. Yung, A. J. Schadock-Hewitt, N. P. Hunter, F. V. Bright, and G. A. Baker, Chem. Commun., 2011, 47, 4775.

    Article  CAS  Google Scholar 

  13. H. Rodríguez, M. Williams, J. S. Wilkes, and R. D. Rogers, Green Chem., 2008, 10, 501.

    Article  Google Scholar 

  14. N. Aoyagi, K. Shimojo, N. R. Brooks, R. Nagaishi, H. Naganawa, K. V. Hecke, L.V. Meervelt, K. Binnemans, and T. Kimura, Chem. Commun., 2011, 47, 4490.

    Article  CAS  Google Scholar 

  15. Y. Qiao, W. Ma, N. Theyssen, C. Chen, and Z. Hou, Chem. Rev., 2011, 117, 6881.

    Article  Google Scholar 

  16. Y. Kohno, S. Saita, Y. Men, J. Yuan, and H. Ohno, Polym. Chem., 2015, 6, 2163.

    Article  CAS  Google Scholar 

  17. B. Monteiro, M. Outis, H. Cruz, J. P. Leal, A. T. Laia, and C. C. L. Pereira, Chem. Commun., 2017, 53, 850.

    Article  CAS  Google Scholar 

  18. A. Branco, L. C. Branco, and F. Pina, Chem. Commun., 2011, 47, 2300.

    Article  CAS  Google Scholar 

  19. A. Branco, J. Belchior, L. C. Branco, and F. Pina, RSC Adv., 2013, 3, 25627.

    Article  CAS  Google Scholar 

  20. A. Kavanagh, K. J. Fraser, R. Byrne, and D. Diamond, ACS Appl. Mater. Interfaces, 2013, 5, 55.

    Article  CAS  PubMed  Google Scholar 

  21. S. V. Muginova, D. A. Myasnikova, S. G. Kazarian, and T. N. Shekhovtsova, Anal. Sci., 2017, 33, 261.

    Article  CAS  PubMed  Google Scholar 

  22. K. Shimojo, Anal. Sci., 2018, 34, 1345.

    Article  CAS  PubMed  Google Scholar 

  23. M. Atanassova, H. Okamura, A. Eguchi, Y. Ueda, T. Sugita, and K. Shimojo, Anal. Sci., 2018, 34, 937.

    Article  Google Scholar 

  24. W. Yoshida, F. Kubota, R. Kono, and M. Goto, Anal. Sci., 2019, 35, 343.

    Article  CAS  PubMed  Google Scholar 

  25. T. Mizuta, K. Sueyoshi, T. Endo, and H. Hisamoto, Sens. Actuators, B, 2018, 258, 1125.

    Article  CAS  Google Scholar 

  26. Y. Niwa, T. Mizuta, K. Sueyoshi, T. Endo, and H. Hisamoto, Analyst, 2019, 144, 6858.

    Article  CAS  PubMed  Google Scholar 

  27. T. Mizuta, K. Sueyoshi, T. Endo, and H. Hisamoto, Bunseki Kagaku, 2019, 68, 945.

    Article  CAS  Google Scholar 

  28. T. Mizuta, K. Maeno, K. Sueyoshi, T. Endo, and H. Hisamoto, Anal. Sci., 2018, 34, 517.

    Article  CAS  PubMed  Google Scholar 

  29. X. Xie and E. Bakker, Anal. Bioanal. Chem., 2015, 407, 3899.

    Article  CAS  PubMed  Google Scholar 

  30. A. Shirai, K. Sueyoshi, T. Endo, and H. Hisamoto, FlatChem, 2019, 13, 34.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was partially supported by the Takahashi Industrial and Economic Research Foundation, the Nakatani Foundation, the Izumi Science and Technology Foundation, the Masuya Memorial Basic Research Foundation, the Sumitomo Foundation, and the Uehara Memorial Foundation.

Author information

Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka, Sakai, Osaka, 599-8531, Japan

    Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo & Hideaki Hisamoto

  2. PRESTO, JST, Japan Science Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Kenji Sueyoshi & Tatsuro Endo

Authors
  1. Ryoutarou Oishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tatsumi Mizuta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kenji Sueyoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tatsuro Endo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hideaki Hisamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hideaki Hisamoto.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Oishi, R., Mizuta, T., Sueyoshi, K. et al. Enzyme-responsive Fluorescent Ionic Liquid. ANAL. SCI. 36, 143–145 (2020). https://doi.org/10.2116/analsci.19C013

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.19C013

Keywords

Search

Navigation