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Highly Sensitive Quantitation of Human Serum Albumin in Clinical Samples for Hypoproteinemia using Metal-Enhanced Fluorescence

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Abstract

In this paper, we have explored metal-enhanced fluorescence (MEF) of the Human serum albumin indicators: Albumin Blue 580, Merocyanine 540 and Bromophenol Blue in close proximity to silver nano-particles, SiFs, from both buffered and clinical samples. The photostability of the Albumin Blue 580 is shown to be much more prolonged from the SiFs as compared to glass (a control sample), potentially allowing for longer detection times to further improve assay statistics. Our findings suggest the widespread use of nanoparticulate SiFs surfaces for the enhanced detection of HSA, particularly for Hypoproteinemia, where an enhanced assay performance at low protein abundance is required.

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Acknowledgement

The authors would like to thank the IoF, Department of Chemistry and Biochemistry and UMBC for salary support.

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Authors and Affiliations

  1. Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 701 East Pratt St, Baltimore, MD, 21202, USA

    Yongxia Zhang, Buddha L. Mali, Caroleann Aitken & Chris D. Geddes

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  1. Yongxia Zhang
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  2. Buddha L. Mali
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  3. Caroleann Aitken
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  4. Chris D. Geddes
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Correspondence to Chris D. Geddes.

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Zhang, Y., Mali, B.L., Aitken, C. et al. Highly Sensitive Quantitation of Human Serum Albumin in Clinical Samples for Hypoproteinemia using Metal-Enhanced Fluorescence. J Fluoresc 23, 187–192 (2013). https://doi.org/10.1007/s10895-012-1133-2

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  • DOI: https://doi.org/10.1007/s10895-012-1133-2

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