Skip to main content
SpringerLink
Log in

Fluorimetric Study on the Interaction between Norfloxacin and Proflavine Hemisulphate

  • Original Paper
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

The interaction between Norfloxacin (NF) and Proflavine hemisulphate (PF) was investigated by spectroscopic tools like UV–VIS absorption and Fluorescence spectroscopy. It was proved that fluorescence quenching of NF by PF is due to the formation of NF-PF complex which was supported by UV–VIS absorption study. The study of thermodynamic parameters suggested that the key interacting forces are hydrogen bond and van der Waal’s interactions and the binding interaction was spontaneous. The distance r between NF and PF was obtained according to the Förster’s theory of non-radiative energy transfer. The fluorescence quenching mechanism was applied to estimate PF directly from pharmaceutical samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

References

  1. Mitchel GAG, Buttle GAH (1943) Proflavine in closed wounds. Lancet 242:749

    Article  Google Scholar 

  2. Nash SC, Ketcham AS, Smith RR (1962) Effect of local irrigation with Proflavine Hemisulfate on wounds seeded with tumor cells an experimental study. Ann Surg 155(3):465–471

    PubMed  CAS  Google Scholar 

  3. Hunt PB, Ford JL (1983) Release of Proflavine hemisulphate from proflavine preparations currently in use within the mersey regional health authority. J Clin Hosp Pharm 8(4):345–348

    PubMed  Google Scholar 

  4. Nelson JM, Chiller TM, Powers JH, Angulo FJ (2007) Food safety: fluoroquinolone-resistant campylobacter species and the withdrawal of fluoroquinolones from use in poultry: a public health success story. Clin Infect Dis 44(7):977–980

    Article  PubMed  CAS  Google Scholar 

  5. Rafalsky VV, Andreeva IV, Rjabkova EL (2006) Quinolones for uncomplicated acute cystitis in women. Cochrane Database Syst Rev 3:CD003597

    PubMed  CAS  Google Scholar 

  6. Drlica K, Zhao X (1997) DNA gyrase, topoisomerase IV and the 4-quinolones. Microbiol Mol Biol Rev 61(3):377–392

    PubMed  CAS  Google Scholar 

  7. More VR, Mote US, Patil SR, Kolekar GB (2010) Fluorescence quenching studies on the interaction between Riboflavin and Norfloxacin and Analytical application on determination of vitamin B2. J Solution Chem 39(1):97–106

    Article  CAS  Google Scholar 

  8. Son GS, Yeo JA, Kim MS, Kim SK, Holmén A, Akerman B, Norden B (1998) Binding mode of Norfloxacin to calf thymus DNA. J Am Chem Soc 120(26):6451–6457

    Article  CAS  Google Scholar 

  9. Song G, He Y, Cai Z (2004) A fluorescence spectroscopic study of the interaction between Norfloxacin and DNA. Can J Anal Sci Spectrosc 49(4):203–209

    CAS  Google Scholar 

  10. More VR, Mote US, Patil SR, Kolekar GB (2009) Spectroscopic studies on the interaction between Norfloxacin and p-Amino benzoic acid: analytical application on determination of Norfloxacin. Spectrochim Acta A 74(3):771–775

    Article  CAS  Google Scholar 

  11. Park HR, Seo JJ, Shin SC, Lee HS, Bark KM (2007) Fluorescence quenching of Norfloxacin by divalent transition metal cations. Bull Korean Chem Soc 28(9):1573–1578

    Article  CAS  Google Scholar 

  12. Ackermann NB, Haldorson DK, Tendik FH, Elslager EF (1968) Preparation and screening of aminoacridines for induction of lung tumor fluorescence in rats. J Med Chem 11(2):315–321

    Article  Google Scholar 

  13. Faller LD, LaFond RE (1971) Binding properties of oligomeric α-chymotrypsin. Biochemistry 10(6):1033–1041

    Article  PubMed  CAS  Google Scholar 

  14. Fernandez-Arguelles MT, Canabate B, Costa-Fernandez JM, Pereiro R, Sanz-Medel A (2004) Flow injection determination of nitrite by fluorescence quenching. Talanta 62(5):991–995

    Article  PubMed  CAS  Google Scholar 

  15. Lee WE, Galley WC (1988) Perturbations to the intersystem crossing of proflavin upon binding to DNA and poly d(A-IU) from triplet-delayed emission spectroscopy. J Biophys 54(4):627–635

    Article  CAS  Google Scholar 

  16. Thomes JC, Weill G, Daune M (1969) Fluorescence of Proflavine–DNA complexes: heterogeneity of binding sites. Biopolymers 8(5):647–659

    Article  PubMed  CAS  Google Scholar 

  17. Chakraborty B, Basu S (2009) Interaction of BSA with Proflavin: a spectroscopic approach. J Lumin 129(1):34–39

    Article  CAS  Google Scholar 

  18. Girousi ST, Alexiadou DK, Ioannou AK (2008) An electroanalytical study of the drug Proflavine. Microchim Acta 160(4):435–439

    Article  CAS  Google Scholar 

  19. Alexiadou DK, Ioannou AK, Kouidou-Andreou S, Voulgaropoulos AN, Girousi ST (2008) Electrochemical study of the interaction mechanism of Proflavine (PF) with DNA using Carbon Paste (CPE) and Hanging Mercury Drop (HMDE) Electrode. Anal Lett 41(10):1742–1750

    Article  CAS  Google Scholar 

  20. Shaw WHC, Wilkinson G (1952) The colorimetric estimation of small quantities of Proflavine hemisulphate. Analyst 77(912):127–132

    Article  CAS  Google Scholar 

  21. Evstigneev MP, Mukhina YV, Davies DB (2006) 1H NMR study of the hetero-association of flavin-mononucleotide with mutagenic dyes: ethidium bromide and proflavine. Mol Phys 104(4):647–654

    Article  CAS  Google Scholar 

  22. Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd edn. Plenum Press, New York

    Book  Google Scholar 

  23. Mote US, Bhattar SL, Patil SR, Kolekar GB (2010) Interaction between felodipine and bovine serum albumin: fluorescence quenching study. Luminescence 25(1):1–8

    PubMed  CAS  Google Scholar 

  24. Bhattar SL, Kolekar GB, Patil SR (2008) Fluorescence resonance energy transfer between perylene and riboflavin in micellar solution and analytical application on determination of vitamin B2. J Lumin 128(3):306–310

    Article  CAS  Google Scholar 

  25. Mote US, Bhattar SL, Patil SR, Kolekar GB (2009) Interaction of fluorescein with felodipine: a spectrofluorometric and thermodynamic study. J Solution Chem 38(5):619–628

    Article  CAS  Google Scholar 

  26. Ross PD, Subramanian S (1981) Thermodynamics of protein association reactions: forces contributing to stability. Biochemistry 20(11):3096–3102

    Article  PubMed  CAS  Google Scholar 

  27. Bhattar SL, Kolekar GB, Patil SR (2010) Spectroscopic studies on the molecular interaction between salicylic acid and riboflavin (B2) in micellar solution. J Lumin 130(3):355–359

    Article  CAS  Google Scholar 

  28. Mote US, Han S-H, Patil SR, Kolekar GB (2010) Effect of temperature and pH on interaction between bovine serum albumin and cetylpyridinium bromide: fluorescence spectroscopic approach. J Lumin 130(11):2059–2064

    Article  CAS  Google Scholar 

Download references

Acknowledgment

One of the authors (VRM) express his gratitude to the Department of Chemistry, The New College, Kolhapur for their kind encouragement.

Author information

Authors and Affiliations

  1. Department of Chemistry, Fluorescence Spectroscopy Research Laboratory, Shivaji University, Kolhapur, 416 004, Maharashtra, India

    Vishalkumar R. More, Prashant V. Anbhule, Shivajirao R. Patil & Govind B. Kolekar

  2. Department of Chemistry, Kyungpook National University, Daegu, South Korea, 133–791

    Sang H. Lee

Authors
  1. Vishalkumar R. More
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Prashant V. Anbhule
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sang H. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shivajirao R. Patil
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Govind B. Kolekar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Govind B. Kolekar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

More, V.R., Anbhule, P.V., Lee, S.H. et al. Fluorimetric Study on the Interaction between Norfloxacin and Proflavine Hemisulphate. J Fluoresc 21, 1789–1796 (2011). https://doi.org/10.1007/s10895-011-0873-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-011-0873-8

Keywords

Search

Navigation