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Laser-Induced Chlorophyll Fluorescence: A Technique for Detection of Dimethoate Effect on Chlorophyll Content and Photosynthetic Activity of Wheat Plant

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Abstract

Laser-induced chlorophyll fluorescence (LICF) spectra and fluorescence induction kinetics (FIK) curves of wheat plant leaves treated with different concentrations (50, 100 and 200 ppm) of dimethoate are recorded. LICF spectra are recorded in the region of 650–780 nm using violet diode laser (405 nm) and FIK curve at 685 and 730 nm with red diode laser (635 nm) for excitation. The fluorescence intensity ratios (FIR) are determined from LICF spectra and vitality index (Rfd) from FIK curves. These parameters along with photosynthetic pigment contents and growth parameters are used to analyze the effect of dimethoate on wheat plants. The result indicates that lower concentration of 50 ppm shows stimulatory response while higher concentrations of dimethoate are hazardous for growth, photosynthetic pigments and activity of wheat plants.

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Acknowledgements

Authors are thankful to Indian Space Research Organization, Bangalore for financial assistance and Dr. B C Tripathy, School of Life Sciences, JNU, New Delhi for his kind suggestions. One of the authors J K Pandey would like to acknowledge UGC, New Delhi for granting fellowship.

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Authors and Affiliations

  1. M.N.Saha Center of Space Studies, IIDS, Nehru Science Center, University of Allahabad, Allahabad, India

    Jitendra Kumar Pandey & R. Gopal

  2. Laser Spectroscopy and Nanomaterial Lab, Department of Physics (UGC-CAS), University of Allahabad, Allahabad, India

    R. Gopal

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  1. Jitendra Kumar Pandey
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  2. R. Gopal
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Correspondence to Jitendra Kumar Pandey.

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Pandey, J.K., Gopal, R. Laser-Induced Chlorophyll Fluorescence: A Technique for Detection of Dimethoate Effect on Chlorophyll Content and Photosynthetic Activity of Wheat Plant. J Fluoresc 21, 785–791 (2011). https://doi.org/10.1007/s10895-010-0771-5

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  • DOI: https://doi.org/10.1007/s10895-010-0771-5

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