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Binding of an Anti-inflammatory Drug Lornoxicam with Blood Proteins: Insights from Spectroscopic Investigations

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Abstract

The interaction between an anti-inflammatory drug, lornoxicam (LXM) and protein (human serum albumin and bovine serum albumin) was studied by spectroscopic techniques (Fluorescence, synchronous, FT-IR, UV-vis absorption and circular dichroism). The quenching mechanism of fluorescence of the protein by the drug was discussed. Based on the interaction studies carried out at different temperatures by spectrofluorometry, the binding constant and the number of binding sites for drug on protein have been evaluated. The nature of binding force operating between the drug and protein was proposed to be electrostatic and hydrophobic based on thermodynamic parameters. The distance r between the donor (protein) and acceptor (drug) was determined based on the Förster’s theory of non-radiation energy transfer and found to be 2.38 nm and 2.56 nm for LXM-BSA and LXM-HSA respectively. Displacement studies with different site probes revealed that the drug bound to the hydrophobic pocket located in sub domain IIA; that is to say, Trp-214 was near or within the binding site. Circular dichroism data of protein in the presence of drug revealed the decreased α-helicity and hence changes in secondary structure of protein. The effects of some common ions were also investigated.

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Acknowledgements

The financial support of the CSIR, New Delhi (No. 01(2279)/08/EMR-II dated 20-11-2008) is gratefully acknowledged. We are thankful to Head, Molecular Biophysics, Indian Institute of Science, Bangalore, for CD measurement facilities. Thanks are also due to the authorities of the Karnatak University, Dharwad, for providing necessary facilities.

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Authors and Affiliations

  1. Department of Chemistry, Karnatak University, Dharwad, 580 003, India

    Reeta Punith, Ashwini H. Hegde & Seetharamappa Jaldappagari

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  1. Reeta Punith
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  2. Ashwini H. Hegde
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  3. Seetharamappa Jaldappagari
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Correspondence to Seetharamappa Jaldappagari.

Additional information

This paper was presented at “Fluorescence 2009”, an International Conference of Fluorescence in Biology, held at Tata Institute of Fundamental Research (TIFR), Mumbai, India, during 16–19th March 2009.

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Punith, R., Hegde, A.H. & Jaldappagari, S. Binding of an Anti-inflammatory Drug Lornoxicam with Blood Proteins: Insights from Spectroscopic Investigations. J Fluoresc 21, 487–495 (2011). https://doi.org/10.1007/s10895-010-0735-9

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  • DOI: https://doi.org/10.1007/s10895-010-0735-9

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