Abstract
A simple and sensitive method for the determination of norepinephrine is described. Norepinephrine (NE) was oxidized by mercury (II) nitrate and the oxidation product was condensed with ethylenediamine (EDA) to form a strong fluorescent compound. The addition of acetone enhances the light intensity. The measurement was carried out at 507 nm with excitation at 420 nm. A linear relationship was obtained between the fluorescence intensity and norepinephrine concentration in the range of 0.01 μM–0.014 mM; the correlation coefficient and the detection limit are 0.99813 and 2.5 nM, respectively. The interference from dopamine (DA) can be eliminated by first derivative synchronous fluorimetric method using peak to zero technique. The recovery efficiency was performed using known amounts of norepinephrine in urine sample and the results indicate a 95–98.62% recovery. The proposed method was also applied to the determination of norepinephrine in injections solution. The reaction mechanism was also described.
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Schreuder WO, Schneider AJ, Groeneveld ABJ, Thijs LG (1989) Effect of dopamine vs norepinephrine on hemodynamics in septic shock. Chest 95:1282–1288
Martin C, Papazian L, Perrin G, Gouin F (1993) Norepinephrine or dopamine for the treatment of hyperdynamic septic shock? Chest 103:1826–1831
Desjars P, Pinaud M, Potel G, Tasseau F, Touze MD (1987) A reappraisal of norepinephrine therapy in human septic shock. Crit Care Med 15:134–137
Moran JL, O’Fathartaigh M, Peisach AR, Chapman MJ, Leppard P (1993) Epinephrine as an inotropic agent in septic shock: a dose profile analysis. Crit Care Med 21:70–77
Jeong H, Kim H, Jeon S (2004) Modified glassy carbon electrode by electropolymerization of tetrakis-(2-aminopheny) porphyrin for the determination of norepinephrine in the presence of ascorbic acid. Microchem J 78:181–186
Yoshitake T, Fujino K, Kehr J, Ishida J, Nohta H, Yamaguchi M (2003) Simultaneous determination of norepinephrine, serotonin, and 5-hydroxyindole-3-acetic acid in microdialysis samples from rat brain by microbore column liquid chromatography with fluorescence detection following derivatization with benzylamine. Anal Biochem 312:125–133
Peterson ZD, Collins DC, Bowerbank CR, Lee ML, Graves SW (2002) Determination of catecholamines and metanephrines in urine by capillary electrophoresis–electrospray ionization–time-of-flight mass spectrometry. J Chromatogr, B 776:221–229
Fotopoulou MA, Ioannou PC (2002) Post-column terbium complexation and sensitized fluorescence detection for the determination of norepinephrine, epinephrine and dopamine using high-performance liquid chromatography. Anal Chim Acta 462:179–185
Kuhlenbeck DL, O’Neill TP, Mack CE, Hoke SH II, Wehmeyer KR (2000) Determination of norepinephrine in small volume plasma samples by stable-isotope dilution gas chromatography-tandem mass spectrometry with negative ion chemical ionization. J Chromatogr, B 738:319–330
Guan CL, Ouyang J, Li QL, Liu BH, Baeyens WRG (2000) Simultaneous determination of catecholamines by ion chromatography with direct conductivity detection. Talanta 50:1197–1203
Ragab GH, Nohta H, Zaitsu K (2000) Chemiluminescence determination of catecholamines in human blood plasma using 1, 2-bis(3-chlorophenyl)ethylenediamine as pre-column derivatizing reagent for liquid chromatography. Anal Chim Acta 403:155–160
Ragab GH, Nohta H, Kai M, Ohkura Y, Zaitsu K (1995) 1, 2-Diarylethylenediamines as sensitive pre-column derivatizing reagents for chemiluminescence detection of catecholamines in HPLC. J Pharm Biomed Anal 13:645–650
Sorouraddin MH, Manzoori JL, Kargarzadeh E, Haji Shabani, AM (1998) Spectrophotometric determination of some catecholamine drugs using sodium bismuthate. J Pharm Biomed Anal 18:877–881
Zhu M, Huang X, Shen H (1997) Peroxidase-based spectrophotometric methods for the determination of ascorbic acid, norepinephrine, epinephrine, dopamine and levodopa. Anal Chim Acta 357:261–267
Karim MM, Jeon CW, Lee HS, Alam SM, Lee SH, Choi JH, Jin SO, Das AK (2006) Simultaneous determination of acetylsalicylic acid and caffeine in pharmaceutical formulation by first derivative synchronous fluorimetric method. J Fluoresc 16:713–721
Tong C, Xiang G (2006) Sensitive determination of norfloxacin by the fluorescence probe of terbium (III)- sodium dodecylbenzene sulfonate and its luminescence mechanism. J Fluoresc 16:831–837
Karim MM, Lee HS, Kim YS, Bae HS, Lee SH (2006) Analysis of salicylic acid based on the fluorescence enhancement of the arsenic (III)-salicylic acid system. Anal Chim Acta 576(1):136–139
Karim MM, Lee SH, Kim YS, Bae HS, Hong S B (2006) Fluorimetric determination of Ce(IV) with ascorbic acid. J Fluoresc 16(1):17–22
Cañizares P, Luque de Castro MD (1995) On-line coupling of isolation/in situ concentration integrated with derivative synchronous spectrofluorimetry for the simultaneous determination of epinephrine and norepinephrine in urine. Anal Chim Acta 317:335–341
Crout JR (1961) In: Selingson D (ed) Standard methods of clinical chemistry, vol 3. Academic, NY
Mabry CC, Warth PW (1969) An automated technic for separate fluorometric measurement of epinephrine and norepinephrine in urine. Am J Clin Pathol 52:57–68
Von Studnitz W (1976) In: Breuer H, Hamel D, Hruskemper HL (eds) Methods of hormone analysis. G. Thieme Verlag, Stuttgart
Yang J, Zhang G, Wu X, Huang F, Lin C, Cao X, Sun L, Ding Y (1998) Fluorimetric determination of epinephrine with o-phenylenediamine. Anal Chim Acta 363:105–110
Wang HY, Hui QS, Xu LX, Jiang JG, Sun Y (2003) Fluorimetric determination of dopamine in pharmaceutical products and urine using ethylene diamine as the fluorigenic reagent. Anal Chim Acta 497:93–99
Liu Y, Yang J, Wu X, Li L (2003) Fluorometric determination of 3, 4-dihydroxyphenylalanine with 2-cyanoacetamide. J Fluoresc 13:123–128
Guo Y, Yang J, Wu X, Du A (2005) A sensitive fluorimetric method for the determination of epinephrine. J Fluoresc 15:131–136
Cohen G, Coldenhero M (1957) The simultaneous fluorimetric determination of adrenaline and noradrenaline in plasma. II. Peripheral venous plasma concentrations in normal subjects and in patients with pheochromocytoma. J Neurochem 2:71–80
Price HL, Price ML (1957) The chemical estimation of epinephrine and norepinephrine in human and canine plasma. II. A critique of the trihydroxyindole method. J Lab Clin Med 50:769–777
Afkhami A, Nematollahi D, Khalafi L, Rafiee M (2005) Kinetic study of the oxidation of some catecholamines by digital simulation of cyclic voltammograms. Int J Chem Kinet 37:17–24
Moldovan Z, Stoica C, Hillebrand M, Alexandrescu L, Macovescu G (2005) Spectrofluorimetric determination of phenyl-β-naphthylamine used as rubber antioxidant. Anal Bioanal Chem 381:1381–1386
Lower SK, El-Sayed MA (1966) The triplet state and molecular electronic processes in organic molecules. Chem Rev 66:199–241
Aksu S, Doyle FM (2002) Electrochemistry of copper in aqueous ethylenediamine solutions. J Electrochem Soc 149(7):B340–B347
Watters JI, Mason JG (1956) Investigation of the complexes of mercury (II) with ethylenediamine using the mercury electrode. J Am Chem Soc 78:285–289
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The support of this research by Korea Research Foundation Grant (KRF-2004-005-C00009) is gratefully acknowledged.
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Karim, M.M., Alam, S.M. & Lee, S.H. Spectrofluorimetric Estimation of Norepinephrine Using Ethylenediamine Condensation Method. J Fluoresc 17, 427–436 (2007). https://doi.org/10.1007/s10895-007-0188-y
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DOI: https://doi.org/10.1007/s10895-007-0188-y