Abstract
A new quantitative method for micro amounts of nucleic acids in aqueous solution is proposed using Eu3+-benzoylacetone (BA) complex as fluorescent probe in the presence of cetyltrimethyl-ammonium bromide (CTMAB). Under the optimum condition, the ratio of the fluorescence intensities with and without nucleic acids is proportional to the concentration of nucleic acid in the range of 1.0 × 10-9 to 5.0 × 10-6 g/mL for herring sperm DNA (hsDNA), 3.0 × 10-9 to 1.0 × 10-6 g/mL for calf thymus DNA(ctDNA) and 8.0 × 10-9 to 1.0 × 10-6 g/mL for yeast RNA (yRNA), and their detection limits are 0.33, 0.21 and 0.99 ng/mL, respectively. Actual sample (DNA of Arabidopsis thaliana) was determined satisfactorily. In addition, the interaction mechanism is also investigated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
S. Udenfriend and P. Zaltzman (1962). Fluorescence characteristic of purines, pyrimidines and their derivatives: Measurement of guanine in nucleic acid hydrolyzates. Anal. Biochem. 3(1), 49–59.
H. C. Borresen (1963). Luminescence properties of some purines and purimidines. Acta Chem. Scand. 17, 921.
W. Y. Li, J. G. Xu, and X. W. He (2000). Determination of nucleic acids using rivanol as the fluorescence probe in the presence of SDS. Anal. Lett. 33(15), 3184–3194.
J. B. LePecq and C. Paoletti (1966). A new fluorometric method for RNA and DNA determination. Anal. Biochem. 17, 100–107.
J. Markovitz, B. P. Roques, and J. B. Le Pecq (1979). Ethidium dimer: A new reagent for the fluorimetric determination of nucleic acids. Anal. Biochem. 94, 259–264.
H. S. Rye, J. M. Dabora, M. A. Quesada, R. A. Mathies, and A. M. Glazer (1993). Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Anal. Biochem. 208, 144–150.
Y. Cao, X. W. He, Z. Gao, and Lu Peng (1999). Fluorescence energy transfer between acridine orange and safranine T and its application in the determination of DNA. Talanta 49, 377–383.
Y. X. Ci, X. L. Hu, Y. Z. Li, and W. B. Chang (1992). Fluorescent reaction between deoxyribonucleic acid, (ribonueleic acid) and terbium(III) and their analytical application. Chin. J. Anal. Chem. 20, 1083–1087.
C. G. Lin, J. H. Yang, X. Wu, G. L. Zhang, R. T. Liu, and R. J. Han (1999). Enhanced fluorescence of the terbium–gadolinium–nucleic acids system and the determination of nucleic acids. Anal. Chim. Acta. 403(1, 2), 219–224.
P. K. Fu and C. Turro (1999). Energy transfer from nucleic acids to Tb(III): Selective emission enhancement by single DNA mismatches. J. Am. Chem. Soc. 121(1), 1–7.
J. H. Yang, C. G. Lin, G. L. Zhang, Z. Q. Gao, X. Wu, and X. R. Huang (1998). Study on a fluorescence-enhanced system of 1,2,4-trihydroxyanthroquinone–Be2+–DNA. Spectrochim. Acta Part A. 54, 2019–2024.
D. H. Li, X. L. Shen, Y. Fang, and J. G. Xu (2001). Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and Acridine Orange. Analyst 126, 518–522.
L. S. Ling, Z. K. He, G. W. Song, H. Y. Han, H. S. Zhang, and Y. E. Zeng (2000). Determination of DNA by use of the molecular “light switch” complex of Ru(bipy)2(dppz)2+. Mirkrochim. Acta. 134, 57–62.
Y. X. Ci, Y. Z. Li, and W. B. Chang (1991). Fluorescence reaction of terbium(III) with nucleic acids in the presence of phenanthroline. Anal. Chim. Acta. 248, 589–594.
Y. X. Ci, Y. Z. Li, and X. J. Liu (1995). Selective Determination of DNA by its enhancement effect on the fluorescence europium-tetracycline complex. Anal. Chem. 67, 1785–1788.
C. Z. Huang, K. A. Li, and S. Y. Tong (1997). Spectrofluorimentric determination of nucleic acids as 8-hydroxyquinoline yttium ternary complexes. Mikrochimica. Acta. 126, 231.
Y. B. Zhao, D. Y. Wang, X. Q. Guo, and J. G. Xu (1997). Determination of DNA and RNA by their quenching effect on the fluorescence of the Tb3+-Tiron complex. Anal. Chim. Acta 353, 329–335.
A. Yegorova, A. Karasyov, A. Duerkop, I. Ukrainets, and V. Antonovich (2005). Igor Ukrainets and Valery Antonovich new luminescent terbium complex for the determination of DNA. Spectrochimica Acta Part A 61, 109–116.
J. H. Yang, H. M. Ge, N. Q. Jie, X. Z. Ren, N. X. Wang, and H. B. Zou (1994). Study on the co-luminescence system Sm-Gd–BPMPHD–CTMAB and its analytical application. Fresenius J. Anal. Chem. 349, 728–733.
J. H. Yang, H. B. Zhou, X. Z. Ren, and C. Y. Li (1990). Fluorescence enhancement of the Eu–Tb-benzoylacetone–phenanthroline system. Anal. Chim. Acta. 238, 307–315.
Y. X. Ci and Z. H. Lan (1989). Fluorometric determination of samarium and gadolinium by enhancement of fluorescence of samarium-thenoyl trifluoroacetone-1,10-phenanthroline ternary complex by gadolinium. Anal.Chem. 61(10), 1063–1069.
J. Sambrook, E. Fritsch, and T. Maintis (1989). Molecular Cloning, a Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York, pp. 9.16–9.19
R. T. Liu, J. H. Yang, Ch. X. Sun, X. Wu, L. Li, and B. Y. Su (2004). Study on the interaction between nucleic acids and cationic surfactants. Coll. Surf. B: Biointerfaces 34, 59–63.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wu, X., Guo, C., Yang, J. et al. The Sensitive Determination of Nucleic Acids Using Fluorescence Enhancement of Eu3+-BenzoylacetoneCetyltrimethylammonium Bromide-Nucleic Acid System. J Fluoresc 15, 655–660 (2005). https://doi.org/10.1007/s10895-005-2971-y
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10895-005-2971-y