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First Observation of Surface Plasmon-Coupled Emission Due to LED Excitation

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Detection limitations for fluorescence methods are normally determined by the phenomenon itself rather than the sensitivity of the instrumentation. These limitations make it necessary to have high sensitivity, high cost equipment causing fluorescence methods to remain lab-oriented. Alleviation of the limitations can be achieved through the phenomenon of surface plasmon-coupled emission (SPCE), which displays enhanced, directional, polarized fluorescence. Here we present the excitation of SPCE from Rhodamine B with a light-emitting diode (LED). Incorporating the gains in sensitivity due to SPCE with LED excitation, it could be possible to design low-cost, high-sensitivity sensors that would allow measurements to be performed in the field.

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ACKNOWLEDGMENTS

This work was made possible by funding from the following grant awards: NSF-BES 0517785 and the National Center for Research Resources, RR-08119. Zygmunt Gryczynski also acknowledges support from Biomolecular Interactions Technologies Center (BITC, New Hampshire).

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Center for Advanced Sensor Technology, University of Maryland at Baltimore County, 5200 Westland Blvd, Baltimore, Maryland, 21227, USA

    Derek S. Smith, Yordan Kostov & Govind Rao

  2. Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland, 21201, USA

    Ignacy Gryczynski, Joanna Malicka, Zygmunt Gryczynski & Joseph R. Lakowicz

Authors
  1. Derek S. Smith
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  2. Yordan Kostov
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  3. Govind Rao
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  4. Ignacy Gryczynski
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  5. Joanna Malicka
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  6. Zygmunt Gryczynski
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  7. Joseph R. Lakowicz
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Correspondence to Govind Rao.

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Smith, D.S., Kostov, Y., Rao, G. et al. First Observation of Surface Plasmon-Coupled Emission Due to LED Excitation. J Fluoresc 15, 895–900 (2005). https://doi.org/10.1007/s10895-005-0021-4

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  • DOI: https://doi.org/10.1007/s10895-005-0021-4

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