Abstract
The use of venom in predation exerts a corresponding selection pressure for the evolution of venom resistance. One of the mechanisms related to venom resistance in animals (predators or prey of snakes) is the presence of molecules in the blood that can bind venom toxins, and inhibit their pharmacological effects. One such toxin type are venom phospholipase A2s (PLA2s), which have diverse effects including anticoagulant, myotoxic, and neurotoxic activities. BoaγPLI isolated from the blood of Boa constrictor has been previously shown to inhibit venom PLA2s that induced myotoxic and edematogenic activities. Recently, in addition to its previously described and very potent neurotoxic effect, the venoms of American coral snakes (Micrurus species) have been shown to have anticoagulant activity via PLA2 toxins. As coral snakes eat other snakes as a major part of their diet, neonate Boas could be susceptible to predation by this sympatric species. Thus, this work aimed to ascertain if BoaγPLI provided a protective effect against the anticoagulant toxicity of venom from the model species Micrurus laticollaris in addition to its ability shown previously against other toxin types. Using a STA R Max coagulation analyser robot to measure the effect upon clotting time, and TEG5000 thromboelastographers to measure the effect upon clot strength, we evaluated the ability of BoaγPLI to inhibit M. laticollaris venom. Our results indicate that BoaγPLI is efficient at inhibiting the M. laticollaris anticoagulant effect, reducing the time of coagulation (restoring them closer to non-venom control values) and increasing the clot strength (restoring them closer to non-venom control values). These findings demonstrate that endogenous PLA2 inhibitors in the blood of non-venomous snakes are multi-functional and provide broad resistance against a myriad of venom PLA2-driven toxic effects including coagulotoxicity, myotoxicity, and neurotoxicity. This novel form of resistance could be evidence of selective pressures caused by predation from venomous snakes and stresses the need for field-based research aimed to expand our understanding of the evolutionary dynamics of such chemical arms race.
Similar content being viewed by others
Data Availability
All data is included in the manuscript figures and raw data is present in Supplementary File 1.
Code Availability
Not applicable.
References
Arbuckle K, Rodríguez de la Vega RC, Casewell NR (2017) Coevolution takes the sting out of it: evolutionary biology and mechanisms of toxin resistance in animals. Toxicon 140:118–131. https://doi.org/10.1016/j.toxicon.2017.10.026
Barchan D, Kachalsky S, Neumann D et al (1992) How the mongoose can fight the snake: the binding site of the mongoose acetylcholine receptor. Proc Natl Acad Sci U S A 89:7717–7721. https://doi.org/10.1073/pnas.89.16.7717
Barchan D, Ovadia M, Kochva E, Fuchs S (1995) The binding site of the nicotinic acetylcholine receptor in animal species resistant to β-Bungarotoxin. Biochemistry 34:9172–9176. https://doi.org/10.1021/bi00028a029
Bastos VA, Gomes-Neto F, Perales J et al (2016) Natural inhibitors of snake venom metalloendopeptidases: history and current challenges. Toxins (Basel) 8:250. https://doi.org/10.3390/toxins8090250
Biardi JE, Coss RG (2011) Rock squirrel (Spermophilus variegatus) blood sera affects proteolytic and hemolytic activities of rattlesnake venoms. Toxicon 57:323–331. https://doi.org/10.1016/j.toxicon.2010.12.011
Bittenbinder MA, Zdenek CN, Op Den Brouw B et al (2018) Coagulotoxic cobras: Clinical implications of strong anticoagulant actions of african spitting Naja venoms that are not neutralised by antivenom but are by LY315920 (varespladib). Toxins (Basel) 10:516. https://doi.org/10.3390/toxins10120516
Brodie ED, Feldman CR, Hanifin CT et al (2005) Parallel arms races between garter snakes and newts involving tetrodotoxin as the phenotypic interface of coevolution. J Chem Ecol 31:343–356. https://doi.org/10.1007/s10886-005-1345-x
Campos PC, de Melo LA, Dias GLF, Fortes-Dias CL (2016) Endogenous phospholipase A2 inhibitors in snakes: a brief overview. J Venom Anim Toxins Incl Trop Dis 22:37. https://doi.org/10.1186/s40409-016-0092-5
Carbajal-Saucedo A, López-Vera E, Bénard-Valle M et al (2013) Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae). Toxicon 66:64–74. https://doi.org/10.1016/j.toxicon.2013.02.006
Carbajal-Saucedo A, Floriano R, Belo C et al (2014) Neuromuscular activity of Micrurus laticollaris (Squamata: Elapidae) venom in vitro. Toxins (Basel) 6:359–370. https://doi.org/10.3390/toxins6010359
Casewell NR, Wüster W, Vonk FJ et al (2013) Complex cocktails: the evolutionary novelty of venoms. Trends Ecol Evol 28:219–229
da Costa Neves-Ferreira AG, Valente RH, Perales J, Domont GB (2009) Enzyme inhibitors in reptile venoms and innate immunity to snake venoms. In: Handbook of venoms and toxins of reptiles. CRC Press, pp 257–276
Dashevsky D, Bénard-Valle M, Neri-Castro E et al (2021) Anticoagulant Micrurus venoms: targets and neutralization. Toxicol Lett 337:91–97. https://doi.org/10.1016/j.toxlet.2020.11.010
de Wit CA, Weström BR (1987) Venom resistance in the Hedgehog, Erinaceus europaeus: purification and identification of macroglobulin inhibitors as plasma antihemorrhagic factors. Toxicon 25:315–323. https://doi.org/10.1016/0041-0101(87)90260-1
Donnini S, Finetti F, Francese S et al (2011) A novel protein from the serum of Python sebae, structurally homologous with type-γ phospholipase A2 inhibitor, displays antitumour activity. Biochem J 440:251–262. https://doi.org/10.1042/BJ20100739
Drabeck DH, Dean AM, Jansa SA (2015) Why the honey badger don’t care: convergent evolution of venom-targeted nicotinic acetylcholine receptors in mammals that survive venomous snake bites. Toxicon 99:68–72. https://doi.org/10.1016/j.toxicon.2015.03.007
Faure G, Xu H, Saul F (2011) Anticoagulant phospholipases A2 which bind to the specific soluble receptor coagulation factor Xa. In: Toxins and hemostasis: from bench to bedside. Springer Netherlands, pp 201–217
Fortes-Dias CL, Fonseca BCB, Kochva E, Diniz CR (1991) Purification and properties of an antivenom factor from the plasma of the South American rattlesnake (Crotalus durissus terrificus). Toxicon 29:997–1008. https://doi.org/10.1016/0041-0101(91)90082-3
Fortes-Dias CL, Campos PC, Fernandes CAH, Fontes MRM (2016) Phospholipase A2 Inhibitors from Snake Blood (sbPLIs). In: Snake Venoms. Springer Netherlands, Dordrecht, pp 1–18
Fortes-Dias CL, Macedo DHF, Barbosa RP et al (2020) Identification and characterization of the first endogenous phospholipase A2 inhibitor from a non-venomous tropical snake, Boa constrictor (Serpentes: Boidae). J Venom Anim Toxins Incl Trop Dis 26:e20190044. https://doi.org/10.1590/1678-9199-jvatitd-2019-0044
Fry BG, Roelants K, Champagne DE et al (2009) The Toxicogenomic multiverse: convergent recruitment of proteins into animal venoms. Annu Rev Genomics Hum Genet 10:483–511. https://doi.org/10.1146/annurev.genom.9.081307.164356
Gibbs HL, Sanz L, Pérez A et al (2020) The molecular basis of venom resistance in a rattlesnake-squirrel predator-prey system. Mol Ecol 29:2871–2888. https://doi.org/10.1111/mec.15529
Gimenes SNC, Lopes DS, Alves PT et al (2017) Antitumoral effects of γcdcPLI, a PLA2 inhibitor from Crotalus durissus collilineatus via PI3K/Akt pathway on MDA-MB-231 breast cancer cell. Sci Rep 7:7077. https://doi.org/10.1038/s41598-017-07082-2
Harris RJ, Fry BG (2021) Electrostatic resistance to alpha-neurotoxins conferred by charge reversal mutations in nicotinic acetylcholine receptors. Proc Biol Sci 288:20202703. https://doi.org/10.1098/rspb.2020.2703
Holding ML, Drabeck DH, Jansa SA, Gibbs HL (2016a) Venom resistance as a model for understanding the molecular basis of complex coevolutionary adaptations. Integr Comp Biol 56(5):1032–1043. https://doi.org/10.1093/icb/icw082
Holding ML, Biardi JE, Gibbs HL (2016b) Coevolution of venom function and venom resistance in a rattlesnake predator and its squirrel prey. Proc R Soc B Biol Sci 283:20152841. https://doi.org/10.1098/rspb.2015.2841
Jones L, Harris RJ, Fry BG (2021) Not goanna get me: mutations in the savannah monitor lizard (Varanus exanthematicus) nicotinic acetylcholine receptor confer reduced susceptibility to sympatric cobra venoms. Neurotox Res. https://doi.org/10.1007/s12640-021-00351-z
Khan MA, Dashevsky D, Kerkkamp H et al (2020) Widespread evolution of molecular resistance to snake venom α-neurotoxins in vertebrates. Toxins (Basel) 12:638. https://doi.org/10.3390/toxins12100638
Kini RM (2003) Excitement ahead: structure, function and mechanism of snake venom phospholipase A2 enzymes. Toxicon 42:827–840. https://doi.org/10.1016/j.toxicon.2003.11.002
Lizano S, Domont G, Perales J (2003) Natural phospholipase A2 myotoxin inhibitor proteins from snakes, mammals and plants. Toxicon 42:963–977. https://doi.org/10.1016/j.toxicon.2003.11.007
Lomonte B, Rey-Suárez P, Fernández J et al (2016) Venoms of Micrurus coral snakes: evolutionary trends in compositional patterns emerging from proteomic analyses. Toxicon 122:7–25. https://doi.org/10.1016/j.toxicon.2016.09.008
McGlothlin JW, Chuckalovcak JP, Janes DE et al (2014) Parallel evolution of tetrodotoxin resistance in three voltage-gated sodium channel genes in the garter snake Thamnophis sirtalis. Mol Biol Evol 31:2836–2846. https://doi.org/10.1093/molbev/msu237
Oliveira CZ, Menaldo DL, Marcussi S et al (2008) An α-type phospholipase A2 inhibitor from Bothrops jararacussu snake plasma: Structural and functional characterization. Biochimie 90:1506–1514. https://doi.org/10.1016/j.biochi.2008.05.009
Oliveira CZ, Santos-Filho NA, Menaldo DL et al (2011) Structural and functional characterization of a γ-type phospholipase A2 inhibitor from Bothrops jararacussu Snake Plasma. Curr Top Med Chem 11:2509–2519. https://doi.org/10.2174/156802611797633465
Rocha SLG, Lomonte B, Neves-Ferreira AGC et al (2002) Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum. Eur J Biochem 269:6052–6062. https://doi.org/10.1046/j.1432-1033.2002.03308.x
Rodrigues CFB, Serino-Silva C, de Morais-Zani K et al (2020) BoaγPLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor. PLoS ONE 15:e0229657. https://doi.org/10.1371/journal.pone.0229657
Roze J (1996) Coral snakes of the Americas: biology, identification, and venoms. Krieger Publishing Company
Saikia D, Mukherjee AK (2017) Anticoagulant and membrane damaging properties of snake venom phospholipase A2 enzymes. In: Snake venoms. Springer Netherlands, Dordrecht, pp 87–104
Samy RP, Gopalakrishnakone P, Chow VTK et al (2012) Therapeutic application of natural inhibitors against snake venom phospholipase A2. Bioinformation 8:48–57. https://doi.org/10.6026/97320630008048
Santos-Filho NA, Fernandes CAHH, Menaldo DL et al (2011) Molecular cloning and biochemical characterization of a myotoxin inhibitor from Bothrops alternatus snake plasma. Biochimie 93:583–592. https://doi.org/10.1016/j.biochi.2010.11.016
Schendel V, Rash LD, Jenner RA, Undheim EAB (2019) The diversity of venom: the importance of behavior and venom system morphology in understanding its ecology and evolution. Toxins (Basel) 11(11):666. https://doi.org/10.3390/toxins11110666
Shirai R, Toriba M, Hayashi K et al (2009) Identification and characterization of phospholipase A2 inhibitors from the serum of the Japanese rat snake, Elaphe climacophora. Toxicon 53:685–692. https://doi.org/10.1016/j.toxicon.2009.02.001
Takacs Z, Wilhelmsen KC, Sorota S (2004) Cobra (Naja spp.) nicotinic acetylcholine receptor exhibits resistance to Erabu sea snake (Laticauda semifasciata) short-chain α-neurotoxin. J Mol Evol 58:516–526. https://doi.org/10.1007/s00239-003-2573-8
Takacs Z, Wilhelmsen KC, Sorota S (2001) Snake α-neurotoxin binding site on the Egyptian Cobra (Naja haje) nicotinic acetylcholine receptor is conserved. Mol Biol Evol 18:1800–1809. https://doi.org/10.1093/oxfordjournals.molbev.a003967
Tanaka-Azevedo AM, Tanaka AS, Sano-Martins IS (2003) A new blood coagulation inhibitor from the snake Bothrops jararaca plasma: isolation and characterization. Biochem Biophys Res Commun 308:706–712. https://doi.org/10.1016/S0006-291X(03)01464-5
Thwin MM, Gopalakrishnakone P (1998) Snake envenomation and protective natural endogenous proteins: a mini review of the recent developments (1991–1997). Toxicon 36:1471–1482. https://doi.org/10.1016/S0041-0101(98)00137-8
Thwin MM, Gopalakrishnakone P, ManjunathaKini R et al (2000) Recombinant antitoxic and antiinflammatory factor from the nonvenomous snake Python reticulatus: phospholipase A2 inhibition and venom neutralizing potential. Biochemistry 39:9604–9611. https://doi.org/10.1021/bi000395z
Thwin MM, Satish RL, Chan STF, Gopalakrishnakone P (2002) Functional site of endogenous phospholipase A2 inhibitor from python serum: phospholipase A2 binding and anti-inflammatory activity. Eur J Biochem 269:719–727. https://doi.org/10.1046/j.0014-2956.2001.02711.x
Ujvari B, Casewell NR, Sunagar K et al (2015) Widespread convergence in toxin resistance by predictable molecular evolution. Proc Natl Acad Sci U S A 112:11911–11916. https://doi.org/10.1073/pnas.1511706112
Van Valen L (1973) A new evolutionary theory. Evol Theory 1:1–30
Xiao H, Pan H, Liao K et al (2017) Snake venom PLA2, a promising target for broad-spectrum antivenom drug development. Biomed Res Int 2017:1–10. https://doi.org/10.1155/2017/6592820
Xiong S, Luo Y, Zhong L et al (2017) Investigation of the inhibitory potential of phospholipase A2 inhibitor gamma from Sinonatrix annularis to snake envenomation. Toxicon 137:83–91. https://doi.org/10.1016/J.TOXICON.2017.07.019
Zdenek CN, Youngman NJ, Hay C et al (2020) Anticoagulant activity of black snake (Elapidae: Pseudechis) venoms: Mechanisms, potency, and antivenom efficacy. Toxicol Lett 330:176–184. https://doi.org/10.1016/j.toxlet.2020.05.014
Zhang D, Li J, Sun S, Huang C (2018) The inhibitory effect of saPLIγ a snake sourced PLA2 inhibitor on carrageenan-induced inflammation in mice. Toxicon 151:89–95. https://doi.org/10.1016/j.toxicon.2018.07.002
Funding
This research was funded by Australian Research Council Discovery Project DP190100304; Fundação de Apoio à Pesquisa do Estado de São Paulo (FAPESP 2018/25786–0); and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.
Author information
Authors and Affiliations
Contributions
Conceptualization, B.G.F. and A.M.T.A.; methodology, B.G.F., C.N.Z.; validation, C.F.B.R., C.N.Z.; formal analysis, C.F.B.R., C.N.Z; investigation, C.F.B.R., C.N.Z., C.S.S, K.M.Z., K.F.G., M.B.V., E.N., A.A., B.G.F., A.M.T.A.; resources, B.G.F., A.M.T.A., K.F.G.; data curation, C.F.B.R., C.Z.N.; writing—original draft preparation, C.F.B.R., A.M.T.A., B.F.G.; writing—review and editing, C.F.B.R. C.N.Z., C.S.S., K.M.Z., A.M.T.A., B.G.F.; supervision, C.N.Z., A.M.T.A, B.G.F.; project administration, A.M.T.A., B.G.F.; funding acquisition, C.F.B.R, A.M.T.A., B.G.F. All authors have read and agreed to the published version of the manuscript.
Corresponding author
Ethics declarations
Ethics Approval
Plasma work was done under the Australian Red Cross Research Agreement #18-03QLD-09; University of Queensland Human Ethics Committee Approval #2016000256. The BoaγPLI was purified under the Instituto Butantan Ethics Committee Approval # 6916110917.
Consent to Participate
Not applicable.
Consent for Publication
Not applicable.
Conflict of Interests
The authors declare no conflict of interests.
Supplementary Information
ESM 1
(XLSX 13.0 kb)
Rights and permissions
About this article
Cite this article
Rodrigues, C.F.B., Zdenek, C.N., Serino-Silva, C. et al. BoaγPLI from Boa constrictor Blood is a Broad-Spectrum Inhibitor of Venom PLA2 Pathophysiological Actions. J Chem Ecol 47, 907–914 (2021). https://doi.org/10.1007/s10886-021-01289-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10886-021-01289-4