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Potato, Solanum Tuberosum, Defense Against Colorado Potato Beetle, Leptinotarsa Decemlineata (Say): Microarray Gene Expression Profiling of Potato by Colorado Potato Beetle Regurgitant Treatment of Wounded Leaves

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Abstract

Colorado potato beetle (CPB) is a leading pest of solanaceous plants. Despite the economic importance of this pest, surprisingly few studies have been carried out to characterize its molecular interaction with the potato plant. In particular, little is known about the effect of CPB elicitors on gene expression associated with the plant’s defense response. In order to discover putative CPB elicitor-responsive genes, the TIGR 11,421 EST Solanaceae microarray was used to identify genes that are differentially expressed in response to the addition of CPB regurgitant to wounded potato leaves. By applying a cutoff corresponding to an adjusted P-value of <0.01 and a fold change of >1.5 or <0.67, we found that 73 of these genes are induced by regurgitant treatment of wounded leaves when compared to wounding alone, whereas 54 genes are repressed by this treatment. This gene set likely includes regurgitant-responsive genes as well as wounding-responsive genes whose expression patterns are further enhanced by the presence of regurgitant. Real-time polymerase chain reaction was used to validate differential expression by regurgitant treatment for five of these genes. In general, genes that encoded proteins involved in secondary metabolism and stress were induced by regurgitant; genes associated with photosynthesis were repressed. One induced gene that encodes aromatic amino acid decarboxylase is responsible for synthesis of the precursor of 2-phenylethanol. This is significant because 2-phenylethanol is recognized by the CPB predator Perillus bioculatis. In addition, three of the 16 type 1 and type 2 proteinase inhibitor clones present on the potato microarray were repressed by application of CPB regurgitant to wounded leaves. Given that proteinase inhibitors are known to interfere with digestion of proteins in the insect midgut, repression of these proteinase inhibitors by CPB may inhibit this component of the plant’s defense arsenal. These data suggest that beyond the wound response, CPB elicitors play a role in mediating the plant/insect interaction.

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Acknowledgments

We are indebted to the TIGR Expression Profiling Service for providing and performing the microarrays and the data acquisition, to Y. Lui for assistance with the statistical analyses of the microarray data, and to R. Bennett for providing the potato plants. We thank E. Clark and L. Liska for providing CPB. Finally, we thank S. Seybold and three anonymous reviewers for constructive feedback on earlier versions of this work.

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Authors and Affiliations

  1. Invasive Insect Biocontrol and Behavior Lab, USDA—ARS, BARC-West, 10,300 Baltimore Ave. Bldg 011A, Rm 214, Beltsville, MD, 20705, USA

    Susan D. Lawrence & Nicole G. Novak

  2. Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2E9, Canada

    Chelsea J.-T. Ju & Janice E. K. Cooke

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  1. Susan D. Lawrence
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  2. Nicole G. Novak
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Correspondence to Susan D. Lawrence.

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Lawrence, S.D., Novak, N.G., Ju, C.JT. et al. Potato, Solanum Tuberosum, Defense Against Colorado Potato Beetle, Leptinotarsa Decemlineata (Say): Microarray Gene Expression Profiling of Potato by Colorado Potato Beetle Regurgitant Treatment of Wounded Leaves. J Chem Ecol 34, 1013–1025 (2008). https://doi.org/10.1007/s10886-008-9507-2

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