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Interaction of hemin with quadruplex DNA

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Abstract

A DNA enzyme with peroxidase activity is a G-quadruplex-based DNAzyme formed by hemin and G-quadruplex DNA. Activity of peroxide DNAzymes can be influenced by the structure of quadruplex DNA. In this investigation, the interaction of hemin with T30695 G-quadruplex DNA is evaluated. Molecular dynamic simulation indicates that the binding mode of hemin to G-quadruplex DNA is end-stacking, which is consistent with absorption spectroscopy. Based on fluorescence spectroscopy, hemin ejects thiazole orange from bases of four-strand DNA. Circular dichroism spectra showed that no alteration occurs in this type of DNA structure.

Peroxidase DNAzyme is formed by hemin and G-quadruplex DNA.

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Acknowledgments

Financial support provided by the Research Council of Institute for Advanced Studies in Basic Sciences is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45195-1159, Iran

    Maryam Ghahremani Nasab, Leila Hassani & Sara Mohammadi Nejad

  2. Laboratory of Cell Biology, National Cancer Institute NIH, Bethesda, MD, 20892, USA

    Davood Norouzi

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  1. Maryam Ghahremani Nasab
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  2. Leila Hassani
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  3. Sara Mohammadi Nejad
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  4. Davood Norouzi
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Correspondence to Leila Hassani.

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Ghahremani Nasab, M., Hassani, L., Mohammadi Nejad, S. et al. Interaction of hemin with quadruplex DNA. J Biol Phys 43, 5–14 (2017). https://doi.org/10.1007/s10867-016-9430-7

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  • DOI: https://doi.org/10.1007/s10867-016-9430-7

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