Abstract
Addition of a moderate (1.4 mM) concentration of H2O2 to protozoon Acanthamoeba castellanii cell cultures at different growth phases caused a different response to oxidative stress. H2O2 treatment of exponentially growing cells significantly delayed their growth; however, in mitochondria isolated from these cells, no damage to their bioenergetic function was observed. In contrast, addition of H2O2 to A. castellanii cells approaching the stationary phase did not influence their growth and viability while seriously affecting mitochondrial bioenergetic function. Although mitochondrial integrity was maintained, oxidative damage was revealed in the reduction of cytochrome pathway activity, uncoupling protein activity, and the efficiency of oxidative phosphorylation as well as the membrane potential and the endogenous ubiquinone reduction level of the resting state. An increase in the alternative oxidase protein level and activity as well as an increase in the membranous ubiquinone content were observed in mitochondria isolated from late H2O2-treated cells. For the first time, the regulation of ubiquinone content in the inner mitochondrial membrane is shown to play a role in the response to oxidative stress. A physiological role for the higher activity of the alternative oxidase in response to oxidative stress in unicellular organisms, such as amoeba A. castellanii, is discussed.
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References
Czarna M, Jarmuszkiewicz W (2005) FEBS Lett 579:3136–3140
Czarna M, Sluse FE, Jarmuszkiewicz W (2007) J Bioenerg Biomembranes 39:149–157
Jarmuszkiewicz W, Wagner AM, Wagner MJ, Hryniewiecka L (1997) FEBS Lett 411:110–114
Jarmuszkiewicz W, Sluse-Goffart CM, Hryniewiecka L, Michejda J, Sluse FE (1998) J Biol Chem 273:10174–10180
Jarmuszkiewicz W, Sluse-Goffart CM, Hryniewiecka L, Sluse FE (1999) J Biol Chem 274(33):23198–23202
Jarmuszkiewicz W, Frączyk O, Hryniewiecka L (2001) Acta Biochim Pol 48:729–737
Jarmuszkiewicz W, Sluse FE, Hryniewiecka L, Sluse-Goffart CM (2002) J Bioenerg Biomembr 34:31–40
Jarmuszkiewicz W, Antos N, Swida A, Czarna M, Sluse FE (2004) FEBS Lett 569:178–184
Jarmuszkiewicz W, Czarna M, Sluse FE (2005a) Biochim Biophys Acta 1708(1):71–78
Jarmuszkiewicz W, Swida A, Czarna M, Antos N, Sluse-Goffart CM, Sluse FE (2005b) J Bioenerg Biomembranes 37(2):97–107
Jarmuszkiewicz W, Antos-Krzeminska N, Drachal-Chrul D, Matkovic K, Nobik W, Pieńkowska J, Swida A, Woyda-Ploszczyca AM, Budzinska M (2008) Acta Biochim Pol 55:349–355
Jarmuszkiewicz W, Woyda-Ploszczyca AM, Krzeminska N, Sluse FE (2010) Biochim Biophys Acta 1797:792–799
Kamo N, Muratsugu M, Hongoh R, Kobatake Y (1979) J Membr Biol 49:105–121
Kicinska A, Swida A, Bednarczyk P, Koszela-Piotrowska I, Choma K, Dolowy K, Szewczyk A, Jarmuszkiewicz W (2007) J Biol Chem 282:17433–17441
Papa S, Skulachev VP (1997) Mol Cell Biochem 174:305–319
Popov VN (2003) Biochem Soc Trans 31(6):13–16
Radi R, Bush KM, Freeman BA (1993) Arch Biochem Biophys 300:409–415
Sweetlove LJ, Heazlewood JL, Herald V, Holtzapffel R, Day DA, Leaver CJ, Millar AH (2002) Plant J 32:891–904
Swida A, Czarna M, Woyda-Płoszczyca A, Kicinska A, Sluse FE, Jarmuszkiewicz W (2007) J Bioenerg Biomembr 39(1):109–115
Swida A, Woyda-Ploszczyca A, Jarmuszkiewicz W (2008) Biochem J 413:359–367
Vercesi AE, Borecky J, Maia ID, Arruda P, Cuccovia IM, Chaimovich H (2006) Annu Rev Plant Biol 57:383–404
Vladimirov YA, Olenev VI, Suslova TB, Cheremisina ZP (1980) Adv Lipid Res 17:173–249
Wainright PO, Hinkle G, Sogin ML, Stickel SK (1993) Science 260:340–342
Woyda-Ploszczyca A, Jarmuszkiewicz (2011) Biochim Biophys Acta 1807:42–52
Woyda-Ploszczyca A, Sluse FE, Jarmuszkiewicz W (2009) Biochim Biophys Acta 1787:264–271
Zhang Y, Marcillat O, Giulivi C, Ernster L, Davies KJA (1990) J Biol Chem 265(27):16330–16336
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Andrzej Woyda-Ploszczyca and Agnieszka Koziel contributed equally to this work.
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Woyda-Ploszczyca, A., Koziel, A., Antos-Krzeminska, N. et al. Impact of oxidative stress on Acanthamoeba castellanii mitochondrial bioenergetics depends on cell growth stage. J Bioenerg Biomembr 43, 217–225 (2011). https://doi.org/10.1007/s10863-011-9351-x
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DOI: https://doi.org/10.1007/s10863-011-9351-x