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Mitochondrial bioenergetics and redox state are unaltered in Trypanosoma cruzi isolates with compromised mitochondrial complex I subunit genes

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Abstract

In trypanosomatids the involvement of mitochondrial complex I in NADH oxidation has long been debated. Here, we took advantage of natural Trypanosoma cruzi mutants which present conspicuous deletions in ND4, ND5 and ND7 genes coding for complex I subunits to further investigate its functionality. Mitochondrial bioenergetics of wild type and complex I mutants showed no significant differences in oxygen consumption or respiratory control ratios in the presence of NADH-linked substrates or FADH2-generating succinate. No correlation could be established between mitochondrial membrane potentials and ND deletions. Since release of reactive oxygen species occurs at complex I, we measured mitochondrial H2O2 formation induced by different substrates. Significant differences not associated to ND deletions were observed among the parasite isolates, demonstrating that these mutations are not important for the control of oxidant production. Our data support the notion that complex I has a limited function in T. cruzi.

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Author notes

  1. Julio César Carranza

    Present address: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Ibagué, Colombia

Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Professor Lineu Prestes, 748 CEP 05508-000, São Paulo, SP, Brasil

    Julio César Carranza, Alicia J. Kowaltowski & Bianca Zingales

  2. Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil

    Marco Aurélio G. Mendonça, Thays C. de Oliveira & Fernanda R. Gadelha

Authors
  1. Julio César Carranza
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  2. Alicia J. Kowaltowski
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  3. Marco Aurélio G. Mendonça
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  4. Thays C. de Oliveira
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  5. Fernanda R. Gadelha
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  6. Bianca Zingales
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Correspondence to Bianca Zingales.

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César Carranza, J., Kowaltowski, A.J., Mendonça, M.A.G. et al. Mitochondrial bioenergetics and redox state are unaltered in Trypanosoma cruzi isolates with compromised mitochondrial complex I subunit genes. J Bioenerg Biomembr 41, 299–308 (2009). https://doi.org/10.1007/s10863-009-9228-4

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  • DOI: https://doi.org/10.1007/s10863-009-9228-4

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