Abstract
Previously, we have theoretically studied the possibility of electrical rhythmic entrainment of carrier-mediated ion transporters, and experimentally realized synchronization and acceleration of the Na/K pumping rate in the cell membrane of skeletal muscle fibers by a specially designed synchronization modulation electric field. In these studies we either used cut fibers under a voltage clamp or intact fibers, but in the presence of ion channels blockers. A question remained as to whether the field-induced activation observed in the pump molecules could effectively increase the intracellular ionic concentration and the membrane potential at physiological conditions. In this paper, we studied the effects of the field on intact fibers without any channel blockers. We monitored the field-induced changes in the ionic concentration gradient across the cell membrane and the membrane potential non-invasively by using a fluorescent probe and confocal microscopic imaging techniques. The results clearly show that the entrainment of the pump molecules by the synchronization modulation electric field can effectively increase the ionic concentration gradient, and hence, hyperpolarize the membrane potential.
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Bibliography
Astumian RD (1997) Thermodynamics and kinetics of a Brownian motor. Science 276:917
Astumian RD (2003) Adiabatic pumping mechanism for ion motive ATPases. Phys Rev Lett 91(11):118102(4)
Blank M, Soo L (1989) Bioelectrochem Bioenerg 22:313--322
Blank M, Soo L (1990) Bioelectrochem Bioenergetics 24:56--61
Chen W (2006a) Synchronization modulation of ion-transporter in cell membrane. Phys Rev Lett (submitted, under review)
Chen W (2006b) Voltage-dependence of carrier-mediated ion transport. Phys Rev E (in press)
Chen W, Dando R (2006) Electrical activation of Na/K pumps can increase ionic concentration gradient and membrane resting potential. Biochem Biophys Acta (submitted, under review)
Chen W, Wu WH (2002) The asymmetric, rectifier-like I--V curve of the Na/K pump transient currents in frog skeletal muscle fibers. Bioelectrochemistry 56:199--203
Chen W, Zhang ZS (2006) Synchronization of Na/K pump molecules by a train of squared pulses. J Bioenergetics Biomembranes (in press)
Chen W, Zhang ZS (in press) Repeated stimulations can prolong the refractory period of the voltage-gated Na channels. Biophys J (submitted, under review)
Clausen T (1998) Clinical and therapeutic significance of the Na, K pump. Clin Sci 95:3--17
Clausen T (2003) Na/K pump regulation and skeletal muscle contractility. Physiol Rev 83:1269--1324
Hille B, Campbell DT (1976) An improved Vaseline gap voltage clamp for skeletal muscle fibers. J Gen Physiol 67:265--293
Irving M, Maylie J, Sizto NL, Chandler WK (1987) Intrinsic optical and passive electrical properties of cut frog twitch fibers. J Gen Physiol 89:1--40
Lauger P (1996) Electrogenic pump molecules
Loew LM (1993) Confocal microscopy of potentiometric fluorescent dyes. Meth Cell Biol V38:195--209
Markin VS, Liu DS, Rosenberg MD, Tsong TY (1992) Resonance transduction of low level periodic signals by an enzyme: an oscillatory activation barrier model. Biophys J 61(4):1045--1049
Robertson B, Astumian D (1991) Frequency dependence of catalyzed reactions in a weak oscillating field. J Chem Phys 94(11):7414--7418
Sims PJ, Waggoner AS, Wang CH, Hoffman JF (1974) Studies on the mechanism by which cyanine dye measure membrane potential in red flood cells and phosphatidylcholine vesicles. Biochemistry V13:3315--3330
Teissie J, Tsong TY (1980) Evidence of Voltage-induced channel opening in Na/K ATPase of human erythrocyte membrane. J Membrane Biol 55:133--140
Tsien RY, Waggoner AS (1990) Fluorophors for confocal microcopy, in “Handbook of Confocal Microscopy,” 169--178.
Tsong TY (1990) Electrical modulation of membrane proteins: Enforced conformational oscillations and biological energy and signal transductions. Ann Rev Biophys Biophys Chem 19:83--106
Tsong TY (2002) Na,K-ATPase as A brownian motor: Electric field-induced conformational fluctuation leads to uphill pumping of cation in the absence of ATP. J Biol Phys 28(2):309--325
Waggoner AS (1979) Dye indicators of membrane potential. Annu Rev Biophys Bioeng V8:847--868
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Chen, W., Dando, R. Synchronization modulation of Na/K pump molecules can hyperpolarize the membrane resting potential in intact fibers. J Bioenerg Biomembr 39, 117–126 (2007). https://doi.org/10.1007/s10863-006-9068-4
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DOI: https://doi.org/10.1007/s10863-006-9068-4