In this study, porous bioglass/gelatin/alginate bone tissue engineering scaffolds were fabricated by three-dimensional printing. The compressive strength and in vitro biomineralization properties of the bioglass–gelatin–alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength. In addition, the cells attach and spread on the BG/Gel/SA scaffolds surfaces represents good adhesion and biocompatibility. Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content. Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Huang G, et al. Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment. Chem Rev. 2017;117:12764–850.
Inzana JA, et al. 3D printing of composite calcium phosphate and collagen scaffolds for bone regeneration. Biomaterials. 2014;35:4026–34.
Dreifke MB, Jayasuriya AA, Jayasuriya AC. Current wound healing procedures and potential care. Mater Sci Eng C Mater Biol Appl. 2015;48:651–62.
Zhong Y, et al. Hyaluronic acid-shelled acid-activatable paclitaxel prodrug micelles effectively target and treat CD44-overexpressing human breast tumor xenografts in vivo. Biomaterials. 2016;84:250–61.
Kim E, et al. Spontaneous bone regeneration after surgical extraction of a horizontally impacted mandibular third molar: a retrospective panoramic radiograph analysis. Maxillofac Plast Reconstr Surg. 2019;41:4.
Chansoria P, et al. Ultrasound-assisted biofabrication and bioprinting of preferentially aligned three-dimensional cellular constructs. Biofabrication. 2019;11:035015.
Khan N, et al. Experimental and mechanism study: partially hydrolyzed polyacrylamide gel degradation and deplugging via ultrasonic waves and chemical agents. Ultrason Sonochem. 2019;56:350–60.
Ambekar RS, Kandasubramanian B. Progress in the advancement of porous biopolymer scaffold: tissue engineering application. Ind Eng Chem Res. 2019;58:6163–94.
Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005;26:5474–91.
Lee KY, Mooney DJ. Alginate: properties and biomedical applications. Prog Polym Sci. 2012;37:106–26.
Hong S, et al. 3D printing of highly stretchable and tough hydrogels into complex, cellularized structures. Adv Mater. 2015;27:4035–40.
Motwani SK, et al. Chitosan-sodium alginate nanoparticles as submicroscopic reservoirs for ocular delivery: formulation, optimisation and in vitro characterisation. Eur J Pharm Biopharm. 2008;68:513–25.
Zhou Z, et al. Fabrication and physical properties of gelatin/sodium alginate/hyaluronic acid composite wound dressing hydrogel. J Macromol Sci Part A. 2014;51:318–25.
Li L, et al. 3D-printed ternary SiO2CaO P2O5 bioglass-ceramic scaffolds with tunable compositions and properties for bone regeneration. Ceram Int. 2019;45:10997–1005.
Fiorilli S, et al. Electrophoretic deposition of mesoporous bioactive glass on glass-ceramic foam scaffolds for bone tissue engineering. J Mater Sci Mater Med. 2015;26:5346.
Hench LL. The future of bioactive ceramics. J Mater Sci Mater Med. 2015;26:86.
Jones JR. Review of bioactive glass: from Hench to hybrids. Acta Biomater. 2013;9:4457–86.
Zhang Y, et al. Mesoporous bioactive glass nanolayer-functionalized 3D-printed scaffolds for accelerating osteogenesis and angiogenesis. Nanoscale. 2015;7:19207–21.
Koudehi MF, et al. Preparation and evaluation of novel nano-bioglass/gelatin conduit for peripheral nerve regeneration. J Mater Sci Mater Med. 2014;25:363–73.
Luo H, et al. Constructing a highly bioactive 3D nanofibrous bioglass scaffold via bacterial cellulose-templated sol-gel approach. Mater Chem Phys. 2016;176:1–5.
Navarro M, et al. New macroporous calcium phosphate glass ceramic for guided bone regeneration. Biomaterials. 2004;25:4233–41.
Gloria A, et al. From 3D hierarchical scaffolds for tissue engineering to advanced hydrogel-based and complex devices for in situ cell or drug release. Procedia CIRP. 2016;49:72–5.
Arcos D, Vallet-Regi M. Sol-gel silica-based biomaterials and bone tissue regeneration. Acta Biomater. 2010;6:2874–88.
Sun JY, et al. Highly stretchable and tough hydrogels. Nature. 2012;489:133–6.
Du JK, et al. Mesoporous sulfur-modified iron oxide as an effective Fenton-like catalyst for degradation of bisphenol A. Appl Catal B: Environ. 2016;184:132–41.
Puurunen RL. Surface chemistry of atomic layer deposition: a case study for the trimethylaluminum/water process. J Appl Phys. 2005;97:52.
Zhang Y, et al. Microstructures and properties of high-entropy alloys. Prog Mater Sci. 2014;61:1–93.
Wang HY, Heilshorn SC. Adaptable hydrogel networks with reversible linkages for tissue engineering. Adv Mater. 2015;27:3717–36.
Gong JP. Why are double network hydrogels so tough? Soft Matter. 2010;6:2583–90.
Xu Z, et al. Strong, conductive, lightweight, neat graphene aerogel fibers with aligned pores. ACS Nano. 2012;6:7103–13.
Liu JC, et al. Self-assembling TiO2 nanorods on large graphene oxide sheets at a two-phase interface and their anti-recombination in photocatalytic applications. Adv Funct Mater. 2010;20:4175–81.
Xing RT, et al. An injectable self-assembling collagen-gold hybrid hydrogel for combinatorial antitumor photothermal/photodynamic therapy. Adv Mater. 2016;28:3669–76.
Kokubo T, Kim HM, Kawashita M. Novel bioactive materials with different mechanical properties. Biomaterials. 2003;24:2161–75.
Ahmed J, et al. Mechanical, thermal, structural and barrier properties of crab shell chitosan/graphene oxide composite films. Food Hydrocoll. 2017;71:141–8.
Misra SK, et al. Poly(3-hydroxybutyrate) multifunctional composite scaffolds for tissue engineering applications. Biomaterials. 2010;31:2806–15.
Haritash AK, Kaushik CP. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. J Hazard Mater. 2009;169:1–15.
Wu SL, et al. Biomimetic porous scaffolds for bone tissue engineering. Mater Sci Eng R: Rep. 2014;80:1–36.
Zhu L-y, et al. Degradation characteristics of HAP/CPP/PLLA nanometer composite for scaffold of osteo-tissue engineering. J Lanzhou Univ Technol. 2012;38:10–4.
Hartgerink JD, Beniash E, Stupp SI. Self-assembly and mineralization of peptide-amphiphile nanofibers. Science. 2001;294:1684–8.
Yoshimoto H, et al. A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials. 2003;24:2077–82.
De Yoreo JJ, et al. Crystallization by particle attachment in synthetic, biogenic, and geologic environments. Science. 2015;349:10.
Nudelman F, et al. The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors. Nat Mater. 2010;9:1004–9.
Bleek K, Taubert A. New developments in polymer-controlled, bioinspired calcium phosphate mineralization from aqueous solution. Acta Biomater. 2013;9:6283–321.
Yao QQ, et al. Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation. Biomaterials. 2017;115:115–27.
Bacakova L, et al. Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants. Biotechnol Adv. 2011;29:739–67.
Tian ZQ, Ren B, Wu DY. Surface-enhanced Raman scattering: from noble to transition metals and from rough surfaces to ordered nanostructures. J Phys Chem B. 2002;106:9463–83.
Swatloski RP, et al. Dissolution of cellose with ionic liquids. J Am Chem Soc. 2002;124:4974–5.
Tang SK, Baker GA, Zhao H. Ether- and alcohol-functionalized task-specific ionic liquids: attractive properties and applications. Chem Soc Rev. 2012;41:4030–66.
Hoppe A, Guldal NS, Boccaccini AR. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials. 2011;32:2757–74.
Yang D, et al. Studies on the structural characterization of lignin, hemicelluloses and cellulose fractionated by ionic liquid followed by alkaline extraction from bamboo. Ind Crops Products. 2013;43:141–9.
Shen YH, et al. Interfacial pH: a critical factor for osteoporotic bone regeneration. Langmuir. 2011;27:2701–8.
Liu YP, et al. Carbon-protected bimetallic carbide nanoparticles for a highly efficient alkaline hydrogen evolution reaction. Nanoscale. 2015;7:3130–6.
Li K, et al. The enhanced angiogenic responses to ionic dissolution products from a boron-incorporated calcium silicate coating. Mater Sci Eng C Mater Biol Appl. 2019;101:513–20.
The authors funding support from the National Nature Science Foundation of China (31500762), Guangdong Science and Technology Program (2014B010133001, 2016A010103009), National key research and development plan project (2016YFC1100600).
Conflict of interest
The authors declare that they have no conflict of interest.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ye, Q., Zhang, Y., Dai, K. et al. Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis. J Mater Sci: Mater Med 31, 77 (2020). https://doi.org/10.1007/s10856-020-06413-6