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Pericardial tissue for cardiovascular application: an in-vitro evaluation of established and advanced production processes

  • Biocompatibility Studies
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Abstract

Pericardial tissue is widely used as a biomaterial, especially for cardiovascular application. Tissue processing plays a key role in developing future scaffolds derived from biological material, yet standardized evaluation is still pending. This study presents a comprehensive assessment of different treatment protocols of bovine pericardium and compares those findings to commercially available decellularized bovine (CAB) and equine (CAE) pericardial patches. Native samples were fixed with glutaraldehyde (GA) or decellularized. These decellularized samples were subsequently either treated with GA (DEC-GA) or sterilized (DEC). Treatment effects were assessed by histological evaluation of structural and biomechanical properties. Furthermore, decellularization efficacy and accuracy of the applied sterilization protocol were evaluated. Cell seeding of processed pericardial samples with human endothelial cells constituted as biocompatibility test.GA-fixed tissue revealed structural deterioration, cytotoxicity and opposed to popular believe, GA-treatment did not lead to sterility of the samples. Biomechanical assessment revealed an increase in tensile strength of GA and a decrease of DEC and DEC-GA. DEC samples were successfully sterilized and showed good decellularization results, with a significant decrease in residual DNA. Comparative assessment revealed overall good results of CAE, yet results of CAB varied largely, e.g. decellularization efficacy or tissue thickness. Biocompatibility of DEC, CAB and CAE was confirmed by successful cell adhesion. Substantial differences of native tissue properties were observed, resulting in varying treatment efficacies. This study provides a first overview describing consequential variations among biomaterials and illustrates the necessity of multidimensional assessment and tissue quality management for biological scaffold development.

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Acknowledgements

The authors would like to thank Evelyn Kienle for her contribution to this work. Her previous comprehensive assessment of sterilization methods of bovine pericardium eventually led to the composition of the final method used in this study. Furthermore, we would like to thank Barbara Steinl and Bettina Wimmer for their support in laboratory work. Last but not least, we are grateful to the Department of Neuropathology at LMU Munich for the help regarding sample processing.

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Authors and Affiliations

  1. Department of Cardiac Surgery, Laboratory for Tissue Engineering, Grosshadern Medical Center, Ludwig Maximilian University of Munich, Munich, Germany

    L. Grefen, F. König, M. Grab, C. Hagl & N. Thierfelder

  2. Institute of Medical and Polymer Engineering, Technical University, Munich, Germany

    F. König & M. Grab

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  1. L. Grefen
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Correspondence to L. Grefen.

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Grefen, L., König, F., Grab, M. et al. Pericardial tissue for cardiovascular application: an in-vitro evaluation of established and advanced production processes. J Mater Sci: Mater Med 29, 172 (2018). https://doi.org/10.1007/s10856-018-6186-6

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