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In vitro evaluation of a multispecies oral biofilm over antibacterial coated titanium surfaces

  • Biocompatibility Studies
  • Original Research
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Abstract

Peri-implantitis is an infectious disease that affects the supporting soft and hard tissues around dental implants and its prevalence is increasing considerably. The development of antibacterial strategies, such as titanium antibacterial-coated surfaces, may be a promising strategy to prevent the onset and progression of peri-implantitis. The aim of this study was to quantify the biofilm adhesion and bacterial cell viability over titanium disc with or without antibacterial surface treatment. Five bacterial strains were used to develop a multispecies oral biofilm. The selected species represent initial (Streptococcus oralis and Actinomyces viscosus), early (Veillonella parvula), secondary (Fusobacterium nucleatum) and late (Porphyromonas gingivalis) colonizers. Bacteria were sequentially inoculated over seven different types of titanium surfaces, combining different roughness level and antibacterial coatings: silver nanoparticles and TESPSA silanization. Biofilm formation, cellular viability and bacterial quantification over each surface were analyzed using scanning electron microscopy, confocal microscopy and real time PCR. Biofilm formation over titanium surfaces with different bacterial morphologies could be observed. TESPSA was able to significantly reduce the cellular viability when compared to all the surfaces (p < 0.05). Silver deposition on titanium surface did not show improved results in terms of biofilm adhesion and cellular viability when compared to its corresponding non-coated surface. The total amount of bacterial biofilm did not significantly differ between groups (p > 0.05). TESPSA was able to reduce biofilm adhesion and cellular viability. However, silver deposition on titanium surface seemed not to confer these antibacterial properties.

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Acknowledgements

Dr Nart, Dr Delgado and Mr Vilarrasa from UIC Barcelona would also like to acknowledge the Dentaid Research Centre (Dentaid S.L.) for the technical support and the access to their facilities. The work was supported by Klockner Implant System.

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Authors and Affiliations

  1. Department of Periodontology Faculty of Dentistry, Universitat Internacional de Catalunya, Av. Josep Trueta s/n, Sant Cugat del Vallés, 08195, Barcelona, Spain

    Javi Vilarrasa, Marta Galofré & José Nart

  2. Bioengineering Institute of Technology, Universitat Internacional de Catalunya, Av. Josep Trueta s/n, Sant Cugat del Vallés, 08195, Barcelona, Spain

    Luis M Delgado

  3. Department of Microbiology, Dentaid Research Center, Dentaid S.L., Ronda Can Fatjò 10, Parc Tecnològic del Vallès, 08290, Barcelona, Spain

    Gerard Àlvarez & Vanessa Blanc

  4. Faculty of Dentistry, Universitat Internacional de Cataluny, Av. Josep Trueta s/n, Sant Cugat del Vallés, 08195, Barcelona, Spain

    Deborah Violant & F. Javier Gil

  5. Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering Technical University of Catalonia (UPC), 08019, Barcelona, Spain

    José María Manero

Authors
  1. Javi Vilarrasa
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  2. Luis M Delgado
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  3. Marta Galofré
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  4. Gerard Àlvarez
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  5. Deborah Violant
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  6. José María Manero
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  7. Vanessa Blanc
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  8. F. Javier Gil
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  9. José Nart
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Correspondence to José Nart.

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Vilarrasa, J., Delgado, L.M., Galofré, M. et al. In vitro evaluation of a multispecies oral biofilm over antibacterial coated titanium surfaces. J Mater Sci: Mater Med 29, 164 (2018). https://doi.org/10.1007/s10856-018-6168-8

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  • DOI: https://doi.org/10.1007/s10856-018-6168-8

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