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HRP-mediated graft polymerization of acrylic acid onto silk fibroins and in situ biomimetic mineralization

  • Biomaterials Synthesis and Characterization
  • Original Research
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Abstract

Silk fibroin (SF) can be extensively utilized in biomedical areas owing to its appreciable bioactivity. In this study, biocompatible composites of SF and hydroxyapatite (HAp) were fabricated through in situ biomimetic mineralization process. Graft copolymerization of acrylic acid (AA) onto SF was conducted by using the catalytic system of acetylacetone (ACAC), hydrogen peroxide (H2O2) and horseradish peroxidase (HRP), for enhancing the deposition of apatite onto the fibroin chains. Subsequently, biomimetic mineralization of the prepared fibroin-based membrane was performed in Ca/P solutions to synthesize the organized SF/HAp composites. The efficacies of graft copolymerization and biomimetic mineralization were evaluated by means of ATR-FTIR, GPC, EDS-Mapping, XRD and others. The results denoted that AA was successfully graft-copolymerized with fibroin and formed the copolymer of silk fibroin-graft-polyacrylic acid (SF-g-PAA), and the grafting percentage (GP) and grafting efficiency (GE) under the optimal condition reached to 23.2% and 29.4%, respectively. More mineral phases were detected on the surface of SF-g-PAA membrane after mineralization process when compared to that of the untreated fibroin membrane, companying with an improved mechanical property. According to MG-63 cell viability and fluorescent adhesion assays, the mineralized SF-g-PAA composite showed satisfactory biocompatibility and exceptional adhesive effects as well. The synthetized composite of SF-g-PAA/HAp can be potentially applied in the fields of bone tissue engineering.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (51373071, 31771039), Qing Lan Project of Jiangsu Province (SJ2016-15), the Fundamental Research Funds for the Central Universities (JUSRP51717A), and the Program for Changjiang Scholars and Innovative Research Teams in Universities (IRT_15R26).

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Authors and Affiliations

  1. Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Buguang Zhou, Qian Zhou, Ping Wang, Jiugang Yuan, Yuanyuan Yu, Qiang Wang & Xuerong Fan

  2. Wuxi Medical School, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Chao Deng

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  1. Buguang Zhou
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Correspondence to Ping Wang.

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Zhou, B., Zhou, Q., Wang, P. et al. HRP-mediated graft polymerization of acrylic acid onto silk fibroins and in situ biomimetic mineralization. J Mater Sci: Mater Med 29, 72 (2018). https://doi.org/10.1007/s10856-018-6084-y

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