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Phosphorylation of Silk Fibroin via Maillard Reaction and Its Behavior of Biomimetic Mineralization

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Abstract

Silk fibroin (SF) has the characteristics of non-toxicity, good biocompatibility and low immunogenicity, and exhibits many potential applications in biomaterial fields. In the present work, chemical phosphorylation of SF was carried out via the Maillard reaction using D-glucose-6-phosphate (GP), aiming at increasing the number of binding-sites for calcium ion and promoting the biomimetic mineralization of SF films. Changes in the molecular weight of SF before and after GP grafting were analyzed by SDS-PAGE and SEC chromatogram. Structures, morphologies, and elemental compositions for the fibroin-based films before and after mineralization were evaluated by the means of EDS, SEM, FTIR, and XRD. The results indicated that GP was successfully grafted onto SF surfaces, companying with a slight decrease in the molecular weight. Biomimetic mineralization led to a noticeable deposition of hydroxyapatite (HAp) on the film of SF-g-GP, and higher contents of calcium and phosphorous were detected on the mineralized material, revealing that phosphorylation promoted the mineralization processing. Meanwhile, biocompatibility of the obtained SF-g-GP/HAp was above the acceptable level according to ISO 10993-5-2009. The present work provides an alternative to prepare the fibroin-based bone repair materials.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31771039, 51373071), the Fundamental Research Funds for the Central Universities (JUSRP51717A), the 111 Project (B17021) and Qing Lan Project of Jiangsu Province (SJ2016-15).

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Authors and Affiliations

  1. Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Jingjing Yuan, Qian Zhou, Ping Wang, Jiugang Yuan & Qiang Wang

  2. Wuxi Medical School, Jiangnan University, Wuxi, 214122, China

    Chao Deng

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  1. Jingjing Yuan
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  2. Qian Zhou
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  3. Ping Wang
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  4. Chao Deng
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  5. Jiugang Yuan
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Correspondence to Ping Wang.

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Yuan, J., Zhou, Q., Wang, P. et al. Phosphorylation of Silk Fibroin via Maillard Reaction and Its Behavior of Biomimetic Mineralization. Fibers Polym 20, 1616–1623 (2019). https://doi.org/10.1007/s12221-019-9062-1

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  • DOI: https://doi.org/10.1007/s12221-019-9062-1

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