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Post implantation fate of adipogenic induced mesenchymal stem cells on Type I collagen scaffold in a rat model

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE. Cell components within the construct were identified 21 days post implantation to delineate cell survival, proliferation & terminal roles in vivo. ASC’s are multipotent, while collagen type I is a natural extra cellular matrix component. Commercially available bovine type I collagen was characterized for its physiochemical properties and cyto-compatibility. Nile red staining of induced ASCs identified red globular structures in cell cytoplasm indicating oil droplet accumulation. Similarly, in vivo implantation of the cell seeded collagen construct in rat model for 21 days in the dorsal muscle, showed genesis of chicken wire network of fat-like cells, which was demonstrated histologically using a variety of staining techniques. Furthermore, fluorescent in situ hybridization (FISH) technique established the efficiency of transplantation wherein the male donor cells with labeled Y chromosome was identified 21 days post implantation from female rat model. Retrieved samples at 21 days indicated adipogenesis in situ, with donor cells highlighted via FISH. The study provides an insight to stem cells in ATE from genesis to functionalization.

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Acknowledgements

The authors acknowledge the Director SCTIMST, Head, BMT for fund and facilities to carry out the work and for the fellowship for BV. Dr. Anil Kumar TV, Mr. Thulasidharan for cLSM and histology. Dr. HK Varma, Dr. Suresh babu and Mr. Sanoj for SEM and FTIR facilities. There is no conflict of interest raised by the authors.

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Authors and Affiliations

  1. Division of Tissue Culture, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India

    Balu Venugopal

  2. Transmission Electron Microscopy Lab, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India

    Francis B. Fernandez & Annie John

  3. Division of Laboratory Animal Science, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India

    V. S. Harikrishnan

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  1. Balu Venugopal
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Correspondence to Annie John.

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Venugopal, B., Fernandez, F., Harikrishnan, V.S. et al. Post implantation fate of adipogenic induced mesenchymal stem cells on Type I collagen scaffold in a rat model. J Mater Sci: Mater Med 28, 28 (2017). https://doi.org/10.1007/s10856-016-5838-7

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  • DOI: https://doi.org/10.1007/s10856-016-5838-7

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