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Triphasic ceramic coated hydroxyapatite as a niche for goat stem cell-derived osteoblasts for bone regeneration and repair

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Abstract

Current treatment strategies for the repair or replacement of bone use synthetic implants with stem cells and their progeny––a new approach to address unmet medical needs. This study has evaluated the effect of a silica-coated bioactive ceramic, namely HASi in comparison to hydroxyapatite (HA) on the adhesion, proliferation and osteogenic differentiation of goat bone marrow-derived mesenchymal stem cells in vitro in a prolonged culture of 28 days. The cellular activities were significantly enhanced on HASi signifying the role of silica to stimulate osteoblast cells. The fabrication of such a ‘cell-ceramic construct using autologous MSCs’ is aimed for the transplantation to a large bone defect site in the goat femur model which still remains a formidable challenge in Orthopedic surgery.

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Acknowledgement

The authors thank The Director, SCTIMST and The Head, BMT Wing, for the facilities provided, Mr. Suresh Babu S for material preparation, Mr. R. Sreekumar for SEM images, Dr. T. V. Anilkumar for cLSM, Dr. Sachin J. Shenoy for bone marrow aspiration, and CSIR for Senior Research fellowship for Ms. Manitha and DRDO for financial support.

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Authors and Affiliations

  1. Transmission Electron Microscopy Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, 695 012, India

    Manitha B. Nair & Annie John

  2. Bioceramics Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, 695 012, India

    H. K. Varma

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  1. Manitha B. Nair
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  2. H. K. Varma
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  3. Annie John
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Correspondence to Annie John.

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Nair, M.B., Varma, H.K. & John, A. Triphasic ceramic coated hydroxyapatite as a niche for goat stem cell-derived osteoblasts for bone regeneration and repair. J Mater Sci: Mater Med 20 (Suppl 1), 251–258 (2009). https://doi.org/10.1007/s10856-008-3598-8

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  • DOI: https://doi.org/10.1007/s10856-008-3598-8

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