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Low frequency magnetic force augments hepatic differentiation of mesenchymal stem cells on a biomagnetic nanofibrous scaffold

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Abstract

Liver tissue engineering using polymeric nanofibrous scaffold and stem cells holds great promises for treating end-stage liver failures. The aim of this study was to evaluate hepatic trans-differentiation potential of human mesenchymal stem cells (hMSCs) on a biomagnetic electrospun nanofibrous scaffold fabricated from a blend of poly-l-lactide (PLLA), collagen and fibrin-rich blood clot, under the influence of a low frequency magnetic field. The scaffold was characterized for surface properties, biochemical and biomechanical parameters and bio-magnetic behaviour. Cell proliferation assay revealed that the scaffold was suitable for hMSCs adhesion and proliferation. Hepatic trans-differentiation potential of hMSCs was augmented on nanofibrous scaffold in magnetic field exposure group compared to control groups, as evident by strong expression of hepatocyte specific markers, albumin release, urea synthesis and presence of an inducible cytochrome P450 system. Our results conclude that biomagnetic scaffold of PLLA/collagen/blood clot augments hepatic trans-differentiation of hMSCs under magnetic field influence.

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Acknowledgments

We wish to thank Frontier Lifeline hospital’s basic research team for their technical assistance and SAIF, IIT Madras for VSM analysis of nanofibers.

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Authors and Affiliations

  1. Stem Cells and Tissue Engineering Laboratory, Frontier Lifeline Hospital, R-30 C, Ambattur Industrial Estate Road, Mogappair, Chennai, India

    Dillip Kumar Bishi & Kotturathu Mammen Cherian

  2. Department of Engineering Design, Indian Institute of Technology Madras, Chennai, India

    Soma Guhathakurta

  3. Centre for Nanofibers and Nanotechnology, NUSNNI, National University of Singapore, Singapore, Singapore

    Jayarama Reddy Venugopal & Seeram Ramakrishna

Authors
  1. Dillip Kumar Bishi
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  2. Soma Guhathakurta
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  3. Jayarama Reddy Venugopal
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  5. Seeram Ramakrishna
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Correspondence to Soma Guhathakurta.

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Bishi, D.K., Guhathakurta, S., Venugopal, J.R. et al. Low frequency magnetic force augments hepatic differentiation of mesenchymal stem cells on a biomagnetic nanofibrous scaffold. J Mater Sci: Mater Med 25, 2579–2589 (2014). https://doi.org/10.1007/s10856-014-5267-4

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