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Endoscopic application of EGF-chitosan hydrogel for precipitated healing of GI peptic ulcers and mucosectomy-induced ulcers

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Abstract

The gastrointestinal (GI) endoscopy has become a standard diagnostic tool for GI ulcers and cancer. In this study we studied endoscopic application of epidermal growth factor-containing chitosan hydrogel (EGF-CS gel) for treatment of GI ulcer. We hypothesized that directional ulcer-coating using EGF-CS gel via endoscope would precipitate ulcer-healing. EGF-CS gel was directly introduced to the ulcer-region after ulceration in acetic acid-induced gastric ulcer (AAU) and mucosal resection-induced gastric ulcer (MRU) rabbit and pig models. The ulcer dimensions and mucosal thicknesses were estimated and compared with those in the control group. Healing efficacy was more closely evaluated by microscopic observation of the ulcer after histological assays. In the AAU model, the normalized ulcer size of the gel-treated group was 2.3 times smaller than that in the non-treated control group on day 3 after ulceration (P < 0.01). In the MRU model, the normalized ulcer size of the gel-treated group was 5.4 times smaller compared to that in the non-treated control group on day 1 after ulceration (P < 0.05). Histological analysis supported the ability of EGF-CS gel to heal ulcers. The present study suggests that EGF-CS gel is a promising candidate for treating gastric bleeding and ulcers.

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Acknowledgments

This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A111060). The large animal study was supported by the National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorders (NCEED).

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Authors and Affiliations

  1. Utah-Inha DDS and Advanced Therapeutics Research Center, Incheon, 400-712, South Korea

    Jin Hee Maeng, Eunhye Lee & Don Haeng Lee

  2. The Second Research Institute, CG Bio & BioAlpha Inc., Seoul, South Korea

    Jungju Kim

  3. Division of Gastroenterology & Hepatology, Department of Internal Medicine, Inha University Hospital, Incheon, 400-712, South Korea

    Byoung Wook Bang, Hyung Gil Kim & Don Haeng Lee

  4. Department of New Drug Development and NCEED, School of Medicine, Inha University, Incheon, 400-712, South Korea

    Su-Geun Yang

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  1. Jin Hee Maeng
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Correspondence to Don Haeng Lee or Su-Geun Yang.

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Jin Hee Maeng and Byoung Wook Bang contributed equally to this study.

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Maeng, J.H., Bang, B.W., Lee, E. et al. Endoscopic application of EGF-chitosan hydrogel for precipitated healing of GI peptic ulcers and mucosectomy-induced ulcers. J Mater Sci: Mater Med 25, 573–582 (2014). https://doi.org/10.1007/s10856-013-5088-x

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  • DOI: https://doi.org/10.1007/s10856-013-5088-x

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