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Novel alginate coated hydrophobically modified chitosan polyelectrolyte complex for the delivery of BSA

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Abstract

Polysaccharides based polyelectrolyte complex nanoparticles (PCNs) intended for use in the delivery of macromolecules were prepared by the self-assembly of deoxycholic acid hydrophobically modified chitosan (CS-DCA) core and then coated with sodium alginate (ALG) shell. The CS-DCA capable of forming nano-sized self-aggregates in medium was prepared by the grafting of DCA to CS. In order to increase the stability of nanoparticles and prevent burst release of drug in bloodstream, polyanionic ALG was coated on the surface of positively charged CS-DCA nanoparticles to form PCNs. Dynamic light scattering results revealed that the mean diameter of the PCNs was about 330 nm, larger than that of uncoated nanoparticles (~150 nm). The zeta potential was big enough to keep the stability of PCNs (−28 mV); no size change was found even upon 1 month storage. Bovine serum albumin could be easily incorporated into the PCNs with encapsulation efficiency (>44 %) and keep a sustained manner without burst release when exposed to PBS (pH 7.4) at 37 °C. These results suggested that PCNs may be a promising drug carrier for a prolonged and sustained delivery in the bloodstream.

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Acknowledgments

This study is financially supported by the Natural Science Foundation of Guangdong (S2012040008003), Guangzhou Science and Technology Plan Project (No.11C32070752), the Key Project of DEGP (cxzd1109), the Ph.D. Programs Foundation of Ministry of Education of China and the Fundamental Research Funds for the Central Universities (21612327). This research is financially supported by Guangzhou Science and Technology Plan Project (No. 11C32070752).

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Authors and Affiliations

  1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China

    Rui Guo, Lili Chen, Shushan Cai, Zonghua Liu, Yi Zhu, Wei Xue & Yuanming Zhang

  2. Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China

    Rui Guo, Zonghua Liu & Wei Xue

  3. Department of Chemistry, Jinan University, Guangzhou, 510632, China

    Lili Chen, Shushan Cai, Yi Zhu & Yuanming Zhang

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  1. Rui Guo
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  2. Lili Chen
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  3. Shushan Cai
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  4. Zonghua Liu
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  5. Yi Zhu
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  6. Wei Xue
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  7. Yuanming Zhang
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Correspondence to Yuanming Zhang.

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Guo, R., Chen, L., Cai, S. et al. Novel alginate coated hydrophobically modified chitosan polyelectrolyte complex for the delivery of BSA. J Mater Sci: Mater Med 24, 2093–2100 (2013). https://doi.org/10.1007/s10856-013-4977-3

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  • DOI: https://doi.org/10.1007/s10856-013-4977-3

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