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Nanocomposite hydrogels for cartilage tissue engineering: mesoporous silica nanofibers interlinked with siloxane derived polysaccharide

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Abstract

Injectable materials for mini-invasive surgery of cartilage are synthesized and thoroughly studied. The concept of these hybrid materials is based on providing high enough mechanical performances along with a good medium for chondrocytes proliferation. The unusual nanocomposite hydrogels presented herein are based on siloxane derived hydroxypropylmethylcellulose (Si-HPMC) interlinked with mesoporous silica nanofibers. The mandatory homogeneity of the nanocomposites is checked by fluorescent methods, which show that the silica nanofibres dispersion is realized down to nanometric scale, suggesting an efficient immobilization of the silica nanofibres onto the Si-HPMC scaffold. Such dispersion and immobilization are reached thanks to the chemical affinity between the hydrophilic silica nanofibers and the pendant silanolate groups of the Si-HPMC chains. Tuning the amount of nanocharges allows tuning the resulting mechanical features of these injectable biocompatible hybrid hydrogels. hASC stem cells and SW1353 chondrocytic cells viability is checked within the nanocomposite hydrogels up to 3 wt% of silica nanofibers.

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Acknowledgements

Authors thank warmly Dr. P. Bertoncini for help with epi-fluorescence experiences and Dr. C. Vinatier for help with SW1353 cell line and biocompatibility experiences. This work was funded by the Région Pays de la Loire within the BIOREGOS 2 project.

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Authors and Affiliations

  1. Institut des Matériaux Jean Rouxel (IMN), CNRS UMR 6502, Université de Nantes, 2 rue de la Houssinière, B.P. 32229, 44322, Nantes Cedex 3, France

    Nela Buchtová, Olivier Chauvet & Jean Le Bideau

  2. INSERM, UMRS 791, Laboratoire d’Ingéniérie Ostéo-Articulaire et Dentaire (LIOAD), Faculté ďOdontologie, Université de Nantes, 1 place Alexis Ricordeau, BP 84215, 44042, Nantes Cedex 1, France

    Gildas Réthoré, Cécile Boyer, Jérôme Guicheux & Pierre Weiss

  3. Centre Hospitalier, Université de Hôtel-Dieu, 1 place Alexis Ricordeau, BP 84215, 44042, Nantes Cedex 1, France

    Gildas Réthoré, Cécile Boyer, Jérôme Guicheux & Pierre Weiss

  4. CEA, DAM, Le Ripault, 37260, Monts, France

    Frédéric Rambaud, Karine Vallé & Philippe Belleville

  5. Chimie de la Matière Condensée de Paris, CNRS UMR 7574, UPMC Université Paris 06, Collège de France, 11 place Marcellin Berthelot, Bat D, 75231, Paris, France

    Clément Sanchez

Authors
  1. Nela Buchtová
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  2. Gildas Réthoré
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  3. Cécile Boyer
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  5. Frédéric Rambaud
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  6. Karine Vallé
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  7. Philippe Belleville
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  8. Clément Sanchez
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  9. Olivier Chauvet
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  10. Pierre Weiss
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  11. Jean Le Bideau
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Correspondence to Jean Le Bideau.

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Buchtová, N., Réthoré, G., Boyer, C. et al. Nanocomposite hydrogels for cartilage tissue engineering: mesoporous silica nanofibers interlinked with siloxane derived polysaccharide. J Mater Sci: Mater Med 24, 1875–1884 (2013). https://doi.org/10.1007/s10856-013-4951-0

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  • DOI: https://doi.org/10.1007/s10856-013-4951-0

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