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Application of strontium-doped calcium polyphosphate scaffold on angiogenesis for bone tissue engineering

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Abstract

The key factor for regenerating large segmental bone defects through bone tissue engineering is angiogenesis in scaffolds. Attempts to overcome this problem, it is a good strategy to develop a new scaffold with bioactivity to induce angiogenesis in bone tissue engineering. In our previous research, the ability of strontium-doped calcium polyphosphate (SCPP) to stimulate the release of angiogenic growth factors from cultured osteoblasts was studied. This study was performed to determine the ability of SCPP to induce angiogenesis within in vitro co-culture model of human umbilical vein endothelial cells (HUVEC) and osteoblasts co-cultured. The bioactivity of developed scaffolds to induce angiogenesis in vivo was also researched in this paper. Co-cultured model has been developed in vitro and then cultured with SCPP scaffold as well as calcium polyphosphate (CPP) scaffold and hydroxylapatite (HA) scaffold. The results showed that the optimal ratio of HUVEC and osteoblasts co-cultured model for in vitro angiogenesis was 5:1. The model could maintain for more than 35 days when cultured with the scaffold and show the best activity at 21st day. Compared with those in CPP and HA scaffold, the formation of tube-like structure and the expression of platelet endothelial cell adhesion molecule in co-cultured model is better in SCPP scaffold. The in vivo immunohistochemistry staining for VEGF also showed that SCPP had a potential to promote the formation of angiogenesis and the regeneration of bone. SCPP scaffold could be served as a potential biomaterial with stimulating angiogenesis in bone tissue engineering and bone repair.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30870616) and the Scientific and technological project of Sichuan Province (2012SZ0015).

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Authors and Affiliations

  1. College of Polymer Science and Engineering, Sichuan University, Chengdu, 610065, Sichuan, People’s Republic of China

    Zhipeng Gu, Xu Zhang, Fei Liu & Xixun Yu

  2. Department of Oncology, The 452 Hospital of Chinese PLA, Chengdu, 610021, Sichuan, People’s Republic of China

    Li Li

  3. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610065, Sichuan, People’s Republic of China

    Huixu Xie

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  1. Zhipeng Gu
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  2. Huixu Xie
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  3. Li Li
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  4. Xu Zhang
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  5. Fei Liu
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  6. Xixun Yu
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Correspondence to Xixun Yu.

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Gu, Z., Xie, H., Li, L. et al. Application of strontium-doped calcium polyphosphate scaffold on angiogenesis for bone tissue engineering. J Mater Sci: Mater Med 24, 1251–1260 (2013). https://doi.org/10.1007/s10856-013-4891-8

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  • DOI: https://doi.org/10.1007/s10856-013-4891-8

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