Skip to main content

Advertisement

SpringerLink
Log in

Efficacy of chitin-PAA-GTMAC gel in promoting wound healing: animal study

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Acrylic grafted chitin (chitin-PAA) was modified with glycidyltrimethylammonium chloride (GTMAC) with the aim of promoting wound healing. The chitin-PAA-GTMAC gels with different GTMAC contents were compared with the original chitin-PAA gel and Intrasite gel for their efficacy in deep wound healing of Wistar rats. Four full-thickness wounds were made on the dorsal skin of rats and then each was treated with 4 materials; chitin-PAA, chitin-PAA-GTMAC(1:4), chitin-PAA-GTMAC(1:10) and Intrasite gel. During 18 days of treatment, the wounds were visually observed and calculated for wound size using image analysis program. Skin wound tissues of sacrificed rats were processed for routine histological observation and immunohistochemistry of proliferating cell nuclear antigen (PCNA). The wounds covered with the chitin derivatives either with or without GTMAC showed a significant reduction in wound size in day 9 in comparison with day 12 for those covered with Intrasite gel. The faster rate and the better pattern of epidermal development observed in histological study as well as the higher dermal cell proliferation (PCNA expression) also demonstrated the better efficiency in wound healing of the chitin derivatives than Intrasite. The earliest epidermal development of the wounds treated with chitin-PAA-GTMAC (1:4) among the tested materials suggested the most promising of this material for the treatment of full-thickness open wound.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Muzzarelli R, Baldassarre V, Conti F, Ferrara P, Biagini G, Gazzanelli G, Vasi V. Biological activity of chitosan: ultrastructural study. Biomaterials. 1988;9:247–52.

    Article  CAS  Google Scholar 

  2. Kurita K. Chitin and chitosan: functional biopolymers from marine crustaceans. Marine Biotech. 2006;8:203–26.

    Article  CAS  Google Scholar 

  3. Uppanan P, Channasanon S, Veeranondh S, Tanodekaew S. Synthesis of GTMAC modified chitin-PAA gel and evaluation of its biological properties. J Biomed Mater Res Part A. 2011;98:185–91.

    Article  Google Scholar 

  4. Jayakumar R, Prabaharan M, Sudheesh Kumar PT, Nair SV, Tamura H. Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv. 2011;29:322–37.

    CAS  Google Scholar 

  5. Muzzarelli RAA, Guerrieri M, Goteri G, Muzzarelli C, Armeni T, Ghiselli R, Cornelissen M. The biocompatibility of dibutyryl chitin in the context of wound dressings. Biomaterials. 2005;26:5844–54.

    Article  CAS  Google Scholar 

  6. Wang W, Lin S, Xiao Y, Huang Y, Tan Y, Cai L, Li X. Acceleration of diabetic wound healing with chitosan-crosslinked collagen sponge containing recombinant human acidic fibroblast growth factor in healing-impaired STZ diabetic rats. Life Sci. 2008;82:190–204.

    Article  CAS  Google Scholar 

  7. Cho YW, Cho YN, Chung SH, Yoo G, Ko SW. Water-soluble chitin as a wound healing accelerator. Biomaterials. 1999;20:2139–45.

    Article  CAS  Google Scholar 

  8. Bravo R, Frank R, Blundell PA, Macdonald-Bravo H. Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ. Nature. 1987;326:515–7.

    Article  CAS  Google Scholar 

  9. Prelich G, Tan CK, Kostura M, Mathews MB, So AG, Downey KM, Stillman B. Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein. Nature. 1987;326:517–20.

    Article  CAS  Google Scholar 

  10. Shivji MKK, Kenny MK, Wood RD. Proliferating cell nuclear antigen is required for DNA excision repair. Cell. 1992;69:367–74.

    Article  CAS  Google Scholar 

  11. Hall PA, Levison DA, Woods AL, Yu CCW, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R, Waseem NH, Lane DP. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol. 1990;162:285–94.

    Article  CAS  Google Scholar 

  12. Tanodekaew S, Prasitsilp M, Swasdison S, Thavornyutikarn B, Pothsree T, Pateepasen R. Preparation of acrylic grafted chitin for wound dressing application. Biomaterials. 2004;25:1453–60.

    Article  CAS  Google Scholar 

  13. Rattanatayarom W, Wattanasirichaigoon S. Evaluation of dermal irritancy potential of carboxymethyl-chitosan hydrogel and poly(acrylic acid) chitin hydrogel. J Med Assoc Thai. 2007;90:724–9.

    Google Scholar 

  14. Angspatt A, Tanvatcharaphan P, Channasanon S, Tanodekaew S, Chokrungvaranont P, Sirimaharaj W. Comparative study between chitin/polyacrylic acid(PAA) dressing, lipido-colloid absorbent dressing and alginate wound dressing: A pilot study in the treatment of partial thickness wound. J Med Assoc Thai. 2010;93:694–7.

    Google Scholar 

  15. Jones A, Vaughan D. Hydrogel dressings in the management of a variety of wound types: a review. J Ortho Nurs. 2005;9(Suppl. 1):S1–11.

    Article  Google Scholar 

  16. Bale S, Banks V, Haglestein S, Harding KG. A comparison of two amorphous hydrogels in the debridement of pressure sores. J Wound Care. 1998;7:65–8.

    CAS  Google Scholar 

  17. Winter GD. Formation of the scab and the rate of epithelization of superficial wounds in the skin of the young domestic pig. Nature. 1962;193:293–4.

    Article  CAS  Google Scholar 

  18. Purna SK, Babu M. Collagen based dressings: a review. Burns. 2000;26:54–62.

    Article  CAS  Google Scholar 

  19. Usami Y, Okamoto Y, Takayama T, Shigemasa Y, Minami S. Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2. J Biomed Mater Res. 1998;42:517–22.

    Article  CAS  Google Scholar 

  20. Kim JY, Lee JK, Lee TS, Park WH. Synthesis of chitooligosaccharide derivative with quaternary ammonium group and its antimicrobial activity against Streptococcus mutans. Int J Biol Macromol. 2003;32:23–7.

    Article  CAS  Google Scholar 

  21. Seong HS, Whang HS, Ko SW. Synthesis of a quaternary ammonium derivative of chito-oligosaccharide as antimicrobial agent for cellulosic fibers. J Appl Polym Sci. 2000;76:2009–15.

    Article  CAS  Google Scholar 

  22. Lim SH, Hudson SM. Synthesis and antimicrobial activity of a water-soluble chitosan derivative with a fiber-reactive group. Carbohydr Res. 2004;339:313–9.

    Article  CAS  Google Scholar 

  23. Hong SR, Lee SJ, Shim JW, Choi YS, Lee YM, Song KW, Park MH, Nam YS, Lee SI. Study on gelatin-containing artificial skin IV: a comparative study on the effect of antibiotic and EGF on cell proliferation during epidermal healing. Biomaterials. 2001;22:2777–83.

    Article  CAS  Google Scholar 

  24. Hergott GJ, Kalnins VI. Expression of proliferating cell nuclear antigen in migrating retinal pigment epithelial cells during wound healing in organ culture. Exp Cell Res. 1991;195:307–14.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research work was financially supported by National Metal and Materials Technology Center, Thailand.

Author information

Authors and Affiliations

  1. Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Rd., Bangkok, Thailand

    Kajee Pilakasiri, Patamaporn Molee & Duangkamol Sringernyuang

  2. Armed Forces Research Institute of Medical Sciences, 315/6 Ratchawithi Rd., Bangkok, Thailand

    Noppadon Sangjun

  3. National Metal and Materials Technology Center, 114 Thailand Science Park, Paholyothin Rd., Klong 1, Klong Luang, Pathumthani, 12120, Thailand

    Somruethai Channasanon & Siriporn Tanodekaew

Authors
  1. Kajee Pilakasiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patamaporn Molee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Duangkamol Sringernyuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Noppadon Sangjun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Somruethai Channasanon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Siriporn Tanodekaew
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Siriporn Tanodekaew.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pilakasiri, K., Molee, P., Sringernyuang, D. et al. Efficacy of chitin-PAA-GTMAC gel in promoting wound healing: animal study. J Mater Sci: Mater Med 22, 2497–2504 (2011). https://doi.org/10.1007/s10856-011-4420-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-011-4420-6

Keywords

Search

Navigation