Abstract
Amorphous peroxotitantes (APT) are insoluble titanium-based particles that bind a variety of metal compounds with high affinity; these particles could be sequestered locally in a solid phase to deliver metal-based drugs. Previous studies have confirmed the ‘biodelivery’ of metals from metal–APT complexes to fibroblasts, but not monocytes. Our goal in the current study was to use monocytic cytokine secretion to assess delivery of gold or platinum-based compounds from APT to human THP1 monocytes. Cytokine secretion was not triggered by APT alone or metal–APT complexes. In monocytes activated by lipopolysaccharide (LPS), APT alone enhanced or suppressed IL1β or IL6 secretion, yet TNFα secretion was unaffected. Complexes of APT and Au(III) or cis-platin altered LPS-activated IL6 or IL1β secretion most, TNFα least. Our results suggest that the APT deliver metals to monocytes.
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Acknowledgments
The authors thank the University of Washington and the Savannah River National Laboratory LDRD Program for their support of our work. The authors also sincerely thank Ms. Petra Lockwood for her excellent technical help with the assays.
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Wataha, J.C., Hobbs, D.T., Wong, J.J. et al. Titanates deliver metal ions to human monocytes. J Mater Sci: Mater Med 21, 1289–1295 (2010). https://doi.org/10.1007/s10856-009-3941-8
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DOI: https://doi.org/10.1007/s10856-009-3941-8