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Osteogenic activity of MG63 cells on bone-like hydroxyapatite/collagen nanocomposite sponges

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Abstract

The hydroxyapatite/collagen (HAp/Col) sponge with 95% (v/v) porosity was prepared by freeze-drying of a HAp/Col fiber suspension. MG63 cells were seeded onto the HAp/Col sponge and cultured under a pressure/perfusion condition with osteogenic supplements. A collagen (Col) sponge was used as a control. The cells with sponge were examined by a histology, total DNA content and gene expression. The cells showed good attachment and proliferation everywhere in the HAp/Col sponge, while the cells mainly proliferated at the peripheral part of the Col sponge. Thus, total DNA content in the HAp/Col sponges reached 1.8 times greater than that in the Col sponges at Day 21. Further, the cells and extracellular matrix only in the HAp/Col sponge were calcified, although the cells in both sponge evenly expressed osteogenic gene. These results suggest that the HAp/Col sponge could be useful as a scaffold for bone tissue engineering.

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Authors and Affiliations

  1. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan

    Teruaki Yoshida, Yoshihisa Koyama & Kazuo Takakuda

  2. Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan

    Teruaki Yoshida & Masanori Kikuchi

Authors
  1. Teruaki Yoshida
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  2. Masanori Kikuchi
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  3. Yoshihisa Koyama
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  4. Kazuo Takakuda
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Correspondence to Masanori Kikuchi.

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Yoshida, T., Kikuchi, M., Koyama, Y. et al. Osteogenic activity of MG63 cells on bone-like hydroxyapatite/collagen nanocomposite sponges. J Mater Sci: Mater Med 21, 1263–1272 (2010). https://doi.org/10.1007/s10856-009-3938-3

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  • DOI: https://doi.org/10.1007/s10856-009-3938-3

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