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Biocompatibility of individually designed scaffolds with human periosteum for use in tissue engineering

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Abstract

The aim of this study was to evaluate and compare the biocompatibility of computer-assisted designed (CAD) synthetic hydroxyapatite (HA) and tricalciumphosphate (TCP) blocks and natural bovine hydroxyapatite blocks for augmentations and endocultivation by supporting and promoting the proliferation of human periosteal cells. Human periosteum cells were cultured using an osteogenic medium consisting of Dulbecco’s modified Eagle medium supplemented with fetal calf serum, Penicillin, Streptomycin and ascorbic acid at 37°C with 5% CO2. Three scaffolds were tested: 3D-printed HA, 3D-printed TCP and bovine HA. Cell vitality was assessed by Fluorescein Diacetate (FDA) and Propidium Iodide (PI) staining, biocompatibility with LDH, MTT, WST and BrdU tests, and scanning electron microscopy. Data were analyzed with ANOVAs. Results: After 24 h all samples showed viable periosteal cells, mixed with some dead cells for the bovine HA group and very few dead cells for the printed HA and TCP groups. The biocompatibility tests revealed that proliferation on all scaffolds after treatment with eluate was sometimes even higher than controls. Scanning electron microscopy showed that periosteal cells formed layers covering the surfaces of all scaffolds 7 days after seeding. Conclusion: It can be concluded from our data that the tested materials are biocompatible for periosteal cells and thus can be used as scaffolds to augment bone using tissue engineering methods.

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Acknowledgements

We gratefully acknowledge our laboratory technician Gisela Otto, for her assistance with the analytical and cell culture procedures and Sebastian Spath for his excellent technical assistance. The authors thank the European Union for financial support within the framework of the MyJoint Project (FP-6 NEST 028861).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Christian-Albrechts-University of Kiel, Arnold-Heller-Str. 3, Haus 26, 24105, Kiel, Germany

    Stephan T. Becker, Timothy Douglas, Yahya Acil, Jörg Wiltfang & Patrick H. Warnke

  2. Fluid Technology and Microfluidics, University of Rostock, Rostock, Germany

    Hermann Seitz

  3. Epsom and St. Helier University Hospitals NHS Trust and South West London Elective Orthopaedic Centre, University College London, London, UK

    Sureshan Sivananthan

  4. Faculty of Health Sciences and Medicine, Bond University, Gold Coast, QLD, Australia

    Patrick H. Warnke

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  1. Stephan T. Becker
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  2. Timothy Douglas
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  3. Yahya Acil
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Correspondence to Stephan T. Becker.

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Becker, S.T., Douglas, T., Acil, Y. et al. Biocompatibility of individually designed scaffolds with human periosteum for use in tissue engineering. J Mater Sci: Mater Med 21, 1255–1262 (2010). https://doi.org/10.1007/s10856-009-3878-y

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  • DOI: https://doi.org/10.1007/s10856-009-3878-y

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