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Characterisation of a new bioadhesive system based on polysaccharides with the potential to be used as bone glue

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Abstract

Although gluing bone is in theory a very attractive alternative to classical fracture treatment, this method is not yet clinically established due to the lack of an adhesive which would meet all the necessary requirements. We therefore developed a novel two-component bioadhesive system with the potential to be used as a bone adhesive based on biocompatible and degradable biopolymers (chitosan, oxidised dextran or starch). After mixing in water, the two components covalently cross-link by forming a Schiff’s base. By the same mechanism, the glue binds to any other exposed amino group such as for example those exposed in fractured bone, even in the presence of water. Modified chitosan was synthesised from commercially available chitosan by deacetylation and was then reduced in molecular weight by heating in acid. The amount of free amino groups was analysed by IR. The molecular weight was determined by viscosimetry. Starch or dextran were oxidised with periodic acid to generate aldehyde groups, which were quantified by titration. l-Dopa was conjugated to oxidised dextran or starch in analogy to the gluing mechanism of mussels. Biomechanical studies revealed that the new glue is superior to fibrin glue, but has less adhesive strength than cyanoacrylates. In vitro cell testing demonstrated excellent biocompatibility, rendering this glue a potential candidate for clinical use.

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Acknowledgements

This work was supported by Synos-Foundation (Nr. 2003 0107-02). We thank Prof. G. Platz (PCI, University of Bayreuth) for discussion and the possibility to use the Ubbelohde viscosimeter.

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Authors and Affiliations

  1. Friedrich-Baur-Research Institute for Biomaterials, University of Bayreuth, Ludwig-Thoma-Str. 36c, 95440, Bayreuth, Germany

    Bettina Hoffmann, Andreas Kokott & Günter Ziegler

  2. Experimental Surgery and Regenerative Medicine, Department of Surgery, University of Munich (LMU), Nussbaumstr. 20, 80336, München, Germany

    Elias Volkmer, Nicole Sedlmayr, Matthias Schieker & Wolf Mutschler

  3. Institute of Orthopaedic Research and Biomechanics, University of Ulm, Helmholtzstr. 14, 89081, Ulm, Germany

    Peter Augat, Michael Ohnmacht & Lutz Claes

Authors
  1. Bettina Hoffmann
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  2. Elias Volkmer
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  3. Andreas Kokott
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  4. Peter Augat
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  10. Günter Ziegler
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Correspondence to Bettina Hoffmann.

Additional information

Bettina Hoffmann and Elias Volkmer contributed equally to this work.

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Hoffmann, B., Volkmer, E., Kokott, A. et al. Characterisation of a new bioadhesive system based on polysaccharides with the potential to be used as bone glue. J Mater Sci: Mater Med 20, 2001–2009 (2009). https://doi.org/10.1007/s10856-009-3782-5

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  • DOI: https://doi.org/10.1007/s10856-009-3782-5

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