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Evaluation of bone matrix and demineralized bone matrix incorporated PLGA matrices for bone repair

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Abstract

The aim of this study was to evaluate the composite matrices prepared using Poly(lactic-co-glycolic acid)- PLGA (85:15) by incorporating human bone matrix (BM) powder or demineralized bone matrix (DBM) powder with the weight ratio of polymer: BM or DBM (75:25) to apply for bone repair. Murine Bone Marrow Stromal Cell (BMSC) attachment was studied with different time points at 30 min, 1 h, 2 h, 4 h, and 6 h for BM/PLGA, DBM/PLGA and PLGA control matrices. All types of matrices were linearly increased the BMSC attachment with the increase of time. Significantly higher number of BMSCs was attached to the both BM/PLGA and DBM/PLGA matrices after 2 h compared to the controls. If BM or DBM is incorporated into biodegradable PLGA matrices and cultured with BMSCs, those composite matrices could be potentially used for bone tissue engineering applications. In addition, particle migration and handling difficulties in DBM powder in clinical applications eliminate using a PLGA matrix. Furthermore, we have observed that DBM/PLGA matrices were structurally stronger compared to the BM/PLGA or control PLGA matrices when they exposed to physiological environment for 72 days.

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Acknowledgement

The University of Toledo is greatly appreciated by the authors for providing financial support for this research. Authors also would like to acknowledge Dr. Vijay Goel in Bioengineering Department at the University of Toledo for providing freeze-dried bone specimens for this study.

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  1. Department of Orthopaedics, University of Toledo, Health Science Campus, Mail Stop 1094, 3065 Arlington Avenue, Toledo, OH, 43614-5807, USA

    A. Champa Jayasuriya & Nabil A. Ebraheim

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  1. A. Champa Jayasuriya
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  2. Nabil A. Ebraheim
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Correspondence to A. Champa Jayasuriya.

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Champa Jayasuriya, A., Ebraheim, N.A. Evaluation of bone matrix and demineralized bone matrix incorporated PLGA matrices for bone repair. J Mater Sci: Mater Med 20, 1637–1644 (2009). https://doi.org/10.1007/s10856-009-3738-9

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  • DOI: https://doi.org/10.1007/s10856-009-3738-9

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