Abstract
Here, the layer-by-layer technique (LbL) was used to modify glass as model biomaterial with multilayers of chitosan and heparin to control the interaction with MG-63 osteoblast-like cells. Different pH values during multilayer formation were applied to control their physico-chemical properties. In the absence of adhesive proteins like plasma fibronectin (pFN) both plain layers were rather cytophobic. Hence, the preadsorption of pFN was used to enhance cell adhesion which was strongly dependent on pH. Comparing the adhesion promoting effects of pFN with an engineered repeat of the FN III fragment and collagen I which both lack a heparin binding domain it was found that multilayers could bind pFN specifically because only this protein was capable of promoting cell adhesion. Multilayer surfaces that inhibited MG-63 adhesion did also cause a decreased cell growth in the presence of serum, while an enhanced adhesion of cells was connected to an improved cell growth.
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References
K. Anselme, Biomaterials 21, 667–681 (2000). doi:10.1016/S0142-9612(99)00242-2
L. He, A. Dexter, A. Middelberg, Chem. Eng. Sci. 61, 989–1003 (2006). doi:10.1016/j.ces.2005.05.064
T. Groth, Z.M. Liu, M. Niepel, D. Peschel, K. Kirchhof, G. Altankov, N. Faucheux in NATO Science Series. Nanoengineered Systems for Regenerative Medicine, ed. by V. Shastri, G. Altankov (Springer, New York, 2009) (accepted for publication)
T. Groth, Z.M. Liu, in Membranes for the Life Sciences, ed. by K.V. Peinemann, S. Pereira Nunes (Wiley-VCH, Weinheim, 2007), p. 27
N. Krasteva, B. Seifert, M. Hopp, G. Malsch, W. Albrecht, G. Altankov, T. Groth, J. Biomater. Sci. Polym. Ed. 16, 1–22 (2005). doi:10.1163/1568562052843348
G. Boese, C. Trimpert, W. Albrecht, G. Malsch, T. Groth, A. Lendlein, Tissue Eng. 13, 2995–3002 (2007). doi:10.1089/ten.2006.0442
Z. Tang, Y. Wang, P. Podsiadlo, N.A. Kotov, Adv. Mater. 18, 3203–3224 (2006). doi:10.1002/adma.200600113
U. Hersel, C. Dahmen, H. Kessler, Biomaterials 24, 4385–4415 (2003). doi:10.1016/S0142-9612(03)00343-0
S. Roessler, R. Born, D. Scharnweber, H. Worch, A. Sewing, M. Dard, J. Mater. Sci. Mater. Med. 12, 871–877 (2001). doi:10.1023/A:1012807621414
T.A. Petrie, J.R. Capadona, C.D. Reyes, A. Garcia, Biomaterials 27, 5459–5470 (2007). doi:10.1016/j.biomaterials.2006.06.027
I.K. Kang, O.H. Kwon, K.H. Byun, Y. Kim, J. Mater. Sci. Mater. Med. 7, 135–140 (1996). doi:10.1007/BF00121251
B. Seifert, P. Romaniuk, T. Groth, J. Mater. Sci. Mater. Med. 7, 465–469 (1996). doi:10.1007/BF00705426
P.J. Schoen, The Anti-Coagulant Activity of Heparin–Biochemical Studies in Purified Systems (Datawyse, Maastricht, 1991)
R. Pankov, K.M. Yamada, J. Cell. Sci. 115, 3861–3863 (2002). doi:10.1242/jcs.00059
S. Ayad, R. Boot-Handford, M.J. Humphries, K.E. Kadler, A. Shuttleworth, The Extracellular Matrix. Facts Book (Academic Press, London, 1994)
H.P. Tan, Y.H. Gong, L.H. Lao, Z.W. Mao, C.Y. Gao, J. Mater. Sci. Mater. Med. 18, 1961–1968 (2007). doi:10.1007/s10856-007-3095-5
J. Fu, J. Ji, W. Yuan, J. Shen, Biomaterials 26, 6684–6692 (2005). doi:10.1016/j.biomaterials.2005.04.034
J.A. Hubbell, Biomaterials in Tissue Engineering. Biotechnology 13, 565–576 (1995). doi:10.1038/nbt0695-565
T. Boontheekul, D.J. Mooney, Curr. Opin. Biotechnol. 14, 1–7 (2003). doi:10.1016/j.copbio.2003.08.004
G. Decher, Science 277,1232–1237 (1997)
P.T. Hammond, Adv. Mater. 16, 1271–1293 (2004). doi:10.1002/adma.200400760
G. Decher, J.B. Schlenoff, Multilayer Thin Films (Wiley-VCH, Weinheim, 2003)
T. Groth, A. Lendlein, Angew. Chem. Int. Ed. 43, 926–928 (2004). doi:10.1002/anie.200301708
M. Kumar, React. Funct. Polym. 46, 1–27 (2000). doi:10.1016/S1381-5148(00)00038-9
B. Casu, Carbohydr. Eur. 11, 18–21 (1994)
N. Faucheux, R. Schweiss, K. Luetzow, C. Werner, T. Groth, Biomaterials 25, 2721–2730 (2004). doi:10.1016/j.biomaterials.2003.09.069
S. Boddohi, C.E. Killingsworth, M.J. Kipper, Biomacromolecules 9, 2021–2028 (2008). doi:10.1021/bm8002573
D.S. Salloum, J.B. Schlenoff, Biomacromolecules 5, 1089–1096 (2004). doi:10.1021/bm034522t
B. Schoeler, N. Delorme, I. Doench, G.B. Sukhorukov, A. Fery, K. Glinel, Biomacromolecules 7, 2065–2071 (2006). doi:10.1021/bm060378a
D. Yoo, S.S. Shiratori, M. Rubner, Macromolecules 31, 4309–4318 (1998). doi:10.1021/ma9800360
D.E. Discher, P. Janmey, Y. Wang, Science 310, 1139–1143 (2005). doi:10.1126/science.1116995
A. Schneider, G. Francius, R. Obeid, P. Schwinté, J. Hemmerlé, B. Frisch, P. Schaaf, J.P. Voegel, B. Senger, C. Picart, Langmuir 22, 1193–1200 (2006). doi:10.1021/la0521802
C.J. Wilson, R.E. Clegg, D.I. Leavesley, M.J. Pearcy, Tissue Eng. 11, 1–18 (2005). doi:10.1089/ten.2005.11.1
R. Tzoneva, N. Faucheux, T. Groth, Biochim. et. Biophys. Acta–Gen. Subjects 1770, 1538–1547 (2007)
Y. Tamada, Y. Ikada, J. Biomed. Mater. Res. 28, 783–789 (1994). doi:10.1002/jbm.820280705
R.E. Baier, A.E. Meyer, J.R. Natiella, R.R. Natiella, J.M. Carter, J. Biomed. Mater. Res. 18, 337–355 (1984). doi:10.1002/jbm.820180404
G. Altankov, K. Richau, T. Groth, Materialwiss. Engin. 34, 1120–1128 (2003)
M.H. Lee, P. Ducheyne, L. Lynch, D. Boettiger, R.J. Composto, Biomaterials 27, 1907–1916 (2006). doi:10.1016/j.biomaterials.2005.11.003
J. Vitte, A.M. Benoliel, A. Pierres, P. Bongrand, Eur. Cell Mater. 7, 52–63 (2004)
N. Faucheux, R. Tzoneva, M.D. Nagel, T. Groth, Biomaterials 27, 234–245 (2006). doi:10.1016/j.biomaterials.2005.05.076
B.G. Keselowsky, D.M. Collard, A.J. Garcia, J. Biomed. Mater. Res. 66A, 247–259 (2003). doi:10.1002/jbm.a.10537
S.A. Sukhishvili, E. Kharlampieva, V. Izumrudov, Macromolecules 39, 8873–8881 (2006). doi:10.1021/ma061617p
B. Sim, J. Cladera, P. O’Shea, J. Biomed. Mater. Res. 68A, 352–359 (2004). doi:10.1002/jbm.a.20022
L. Bacakova, E. Filova, F. Ripacek, V. Svorcik, V. Stary, Physiol. Res. 53(Suppl. 1), S35–S45 (2004)
Acknowledgements
This work was supported by Graduiertenförderung des Landes Sachsen-Anhalt with a Ph.D. scholarship to KK and the German Academic Exchange Service (DAAD) in the frame of a bilateral exchange program between Germany and Bulgaria. Dr. J. Vogel is acknowledged for scientific support and discussion.
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Kirchhof, K., Hristova, K., Krasteva, N. et al. Multilayer coatings on biomaterials for control of MG-63 osteoblast adhesion and growth. J Mater Sci: Mater Med 20, 897–907 (2009). https://doi.org/10.1007/s10856-008-3639-3
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DOI: https://doi.org/10.1007/s10856-008-3639-3