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Cellular response to titanium discs coated with polyelectrolyte multilayer films

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Abstract

The purpose of this study was to investigate the effects of polyelectrolyte multilayer (PEM) coatings on the biological behavior of titanium (Ti) substrates. Collagen type Ι/hyaluronic acid (Col/HA) and chitosan/hyaluronic acid (Chi/HA) multilayer PEM coatings were introduced onto Ti substrates using layer-by-layer assembly. Contact angle instruments and quartz crystal microbalance were used for film characterization. The results obtained showed that both Col/HA and Chi/HA surfaces had high hydrophilicity and promoted cell adhesion in MC3T3-E1 pre-osteoblast and human gingival fibroblast cells. In addition, the synthesis of function-related proteins and gene expression levels in both MC3T3-E1 and fibroblast cells was higher for the Col/HA coating compared with the Chi/HA coating, indicating better cellular response to the Col/HA coating.

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References

  1. M.M. Stevens and J.H. George, Exploring and engineering the cell surface interface, Science, 310(2005), No. 5751, p. 1135.

    Article  Google Scholar 

  2. L.L. Hench and J.M. Polak, Third-generation biomedical materials, Science, 295(2002), No. 5557, p. 1014.

    Article  Google Scholar 

  3. I.A. Alsarra, Chitosan topical gel formulation in the management of burn wounds, Int. J. Biol. Macromol., 45(2009), No. 1, p. 16.

    Article  Google Scholar 

  4. E.P. Buchanan, M.T. Longaker, and H.P. Lorenz, Fetal skin wound healing, Adv. Clin. Chem., 48(2009), p. 137.

    Article  Google Scholar 

  5. C.J. Park, S.G. Clark, C.A. Lichtensteiger, R.D. Jamison, and A.J. Johnson, Accelerated wound closure of pressure ulcers in aged mice by chitosan scaffolds with and without bFGF, Acta Biomater., 5(2009), No. 6, p. 1926.

    Article  Google Scholar 

  6. S. Suri and C.E. Schmidt, Photopatterned collagen-hyaluronic acid interpenetrating polymer network hydrogels, Acta Biomater., 5(2009), No. 7, p. 2385.

    Article  Google Scholar 

  7. J.Y. Lee, S.H. Nam, S.Y. Im, Y.J. Park, Y.M. Lee, Y.J. Seol, C.P. Chung, and S.J. Lee, Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials, J. Controlled Release, 78(2002), No. 1–3, p. 187.

    Article  Google Scholar 

  8. M. Morra, C. Cassinelli, G. Cascardo, P. Cahalan, L. Cahalan, M. Fini, and R. Giardino, Surface engineering of titanium by collagen immobilization: surface characterization and in vitro and in vivo studies, Biomaterials, 24(2003), No. 25, p. 4639.

    Article  Google Scholar 

  9. M.M. de Villiers, D.P. Otto, S.J. Strydom, and Y.M. Lvov, Introduction to nanocoatings produced by layer-by-layer (LbL) self-assembly, Adv. Drug. Deliv. Rev., 63(2011), No. 9, p. 701.

    Article  Google Scholar 

  10. G.K. Such, A.P. Johnston, and F. Caruso, Engineered hydrogen-bonded polymer multilayers: from assembly to biomedical applications, Chem. Soc. Rev., 40(2011), No. 1, p. 19.

    Article  Google Scholar 

  11. Q.J. Luo, Y. Huang, X.L. Deng, J.X. Zhang, X.H. Li, S.F. Zhao, and X.D. Li, Polyelectrolyte multilayer coating with two regulatory molecules on titanium: construction and its biological effects, Nanomedicine, 8(2013), No. 5, p. 739.

    Article  Google Scholar 

  12. Y. Huang, Q.J. Luo, G.Y. Zha, J.X. Zhang, X.H. Li, S.F. Zhao, and X.D. Li, Biomimetic ECM coatings for controlled release of rhBMP-2: construction and biological evaluation, Biomater. Sci., 2(2014), p. 980.

    Article  Google Scholar 

  13. J.D. Mendelsohn, S.Y. Yang, J. Hiller, A.I. Hochbaum, and M.F. Rubner, Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films, Biomacromolecules, 4(2003), No. 1, p. 96.

    Article  Google Scholar 

  14. J. Zhang, B. Senger, D. Vautier, C. Picart, P. Schaaf, J.C. Voegel, and P. Lavalle, Natural polyelectrolyte films based on layer-by layer deposition of collagen and hyaluronic acid, Biomaterials, 26(2005), No. 16, p. 3353.

    Article  Google Scholar 

  15. P. Tryoen-Tóth, D. Vautier, Y. Haikel, J.C. Voegel, P. Schaaf, J. Chluba, and J. Ogier, Viability, adhesion, and bone phenotype of osteoblast-like cells on polyelectrolyte multilayer films, J. Biomed. Mater. Res., 60(2002), No. 4, p. 657.

    Article  Google Scholar 

  16. Y. Huang, Q.J. Luo, X.D. Li, F. Zhang, and S.F. Zhao, Fabrication and in vitro evaluation of the collagen/hyaluronic acid PEM coating crosslinked with functionalized RGD peptide on titanium, Acta Biomater., 8(2012), No. 2, p. 866.

    Article  Google Scholar 

  17. X.D. Li, J.X. Zhang, Q.L. Hu, and X.H. Li, From the 2-dimensional unstable polyelectrolyte multilayer to the 3-dimensional stable dry polyelectrolyte capsules, J. Colloid Interface Sci., 363(2011), No. 1, p. 64.

    Google Scholar 

  18. X.J. Li, Q.J. Luo, Y. Huang, X.D. Li, F. Zhang, and S.F. Zhao, The responses of preosteoblasts to collagen/hyaluronic acid polyelectrolyte multilayer coating on titanium, Polym. Adv. Technol., 23(2012), No. 4, p. 756.

    Article  Google Scholar 

  19. Y. Lvov, Electrostatic layer-by-layer assembly of proteins and polyions, [in] Protein Architecture: Interfacing Molecular Assemblies and Immobilization Biotechnology, Marcel Dekker, New York, 2000, p. 125.

    Google Scholar 

  20. B.D. Boyan, Z. Schwartz, and J.C. Hambleton, Response of bone and cartilage cells to biomaterials in vivo and in vitro, J. Oral Implantol., 19(1993), No. 2, p. 116.

    Google Scholar 

  21. A. Nanci, J.D. Wuest, L. Peru, P. Brunet, V. Sharma, S. Zalzal, and M.D. McKee, Chemical modification of titanium surfaces for covalent attachment of biological molecules, J. Biomed. Mater. Res., 40(1998), No. 2, p. 324.

    Article  Google Scholar 

  22. K.L. Ou, R.J. Chung, F.Y. Tsai, P.Y. Liang, S.W. Huang, and S.Y. Chang, Effect of collagen on the mechanical properties of hydroxyapatite coatings, J. Mech. Behav. Biomed. Mater., 4(2011), No. 4, p. 618.

    Article  Google Scholar 

  23. E. Zimmerman, B. Geiger, and L. Addadi, Initial stages of cell-matrix adhesion can be mediated and modulated by cell-surface hyaluronan, Biophys. J., 82(2002), No. 4, p. 1848.

    Article  Google Scholar 

  24. U. Geissler, U. Hempel, C. Wolf, D. Scharnweber, H. Worch, and K. Wenzel, Collagen type I-coating of Ti6Al4V promotes adhesion of osteoblasts, J. Biomed. Mater. Res., 51(2000), No. 4, p. 752.

    Article  Google Scholar 

  25. C.T. Brighton and S.M. Albelda, Identification of integrin cell-substratum adhesion receptors on cultured rat bone cells, J. Orthop. Res., 10(1992), No. 6, p. 766.

    Article  Google Scholar 

  26. J.C. Park, H.M. Kim, and J. Ko, Effects of extracellular matrix constituents on the attachment of human oral epithelial cells at the titanium surface, Int. J. Oral Maxillofac. Implants, 13(1998), No. 6, p. 826.

    Google Scholar 

  27. A. Lahiji, A. Sohrabi, D.S. Hungerford, and C.G. Frondoza, Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes, J. Biomed. Mater. Res., 51(2000), No. 4, p. 586.

    Article  Google Scholar 

  28. P.H. Chua, K.G. Neoh, E.T. Kang, and W. Wang, Surface functionalization of titanium with hyaluronic acid/chitosan polyelectrolyte multilayers and RGD for promoting osteoblast functions and inhibiting bacterial adhesion, Biomaterials, 29(2008), No. 10, p. 1412.

    Article  Google Scholar 

  29. G.S. Stein, J.B. Lian, L.G. Gerstenfeld, V. Shalhoub, M. Aronow, T. Owen, and E. Markose, The onset and progression of osteoblast differentiation is functionally related to cellular proliferation, Connect. Tissue Res., 20(1989), No. 1–4, p. 3.

    Article  Google Scholar 

  30. G.R. Beck Jr., E.C. Sullivan, E. Moran, and B. Zerler, Relationship between alkaline phosphatase levels, osteopontin expression, and mineralization in differentiating MC3T3-E1 osteoblasts, J. Cell. Biochem., 68(1998), No. 2, p. 269.

    Article  Google Scholar 

  31. G.S. Stein and J.B. Lian, Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype, Endocr. Rev., 14(1993), No. 4, p. 424.

    Article  Google Scholar 

  32. C.L. Lavelle, Mucosal seal around endosseous dental implants, J. Oral Implantol., 9(1981), No. 3, p. 357.

    Google Scholar 

  33. M. Khadra, The effect of low level laser irradiation on implant-tissue interaction, In vivo and in vitro studies, Swed. Dent. J. Suppl., 2005, No. 172, p. 1.

    Google Scholar 

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Authors and Affiliations

  1. Department of Stomatology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310016, China

    Jing Zhan

  2. Department of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, 310031, China

    Qiao-jie Luo, Ying Huang & Xiao-dong Li

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  1. Jing Zhan
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  4. Xiao-dong Li
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Correspondence to Xiao-dong Li.

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Zhan, J., Luo, Qj., Huang, Y. et al. Cellular response to titanium discs coated with polyelectrolyte multilayer films. Int J Miner Metall Mater 21, 925–933 (2014). https://doi.org/10.1007/s12613-014-0991-9

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  • DOI: https://doi.org/10.1007/s12613-014-0991-9

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