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Growth and phenotypic expression of human endothelial cells cultured on a glass-reinforced hydroxyapatite

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Abstract

Glass-reinforced hydroxyapatite composites (GR–HA) are bone regenerative materials that are characterized by their increased mechanical properties, when compared to synthetic hydroxyapatite. Bonelike® is a GR–HA that is a result of the addition of a CaO–P2O5 based glass to a HA matrix. This biomaterial has been successfully applied in clinical bone regenerative applications. This work aims to evaluate the ability of Bonelike® to support the adhesion, proliferation and phenotypic expression of human endothelial cells, aiming to establish new bone tissue engineering pre-endothelialization strategies. Bonelike® discs, regardless of being submitted to a pre-immersion treatment with culture medium, were seeded with first passage human umbilical vein endothelial cells, and characterized regarding proliferation and differentiation events. Pre-immersed Bonelike® allowed the adhesion, proliferation and phenotype expression of endothelial cells. Seeded materials presented positive immunofluorescent staining for PECAM-1 and a tendency for the formation of cord-like arrangements under angiogenesis-stimulating conditions, although, compared to standard culture plates, a slight decreased cell growth was observed. In this way, Bonelike® may be a suitable candidate for pre-endothelialization approaches in bone tissue engineering applications.

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Acknowledgments

This work was partially supported by Faculdade de Medicina Dentária da Universidade do Porto (FMDUP).

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Authors and Affiliations

  1. Cooperativa de Ensino Superior Egas Moniz, Monte de Caparica, Portugal

    J. M. Silva Marques

  2. Laboratório de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina Dentária, Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393, Porto, Portugal

    P. S. Gomes & M. H. Fernandes

  3. Faculdade de Engenharia, Universidade do Porto, Porto, Portugal

    M. A. Silva & J. D. Santos

  4. Instituto de Patologia Experimental, Faculdade de Medicina, Universidade de Coimbra, Coimbra, Portugal

    A. M. Silvério Cabrita

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  1. J. M. Silva Marques
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Correspondence to M. H. Fernandes.

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Silva Marques, J.M., Gomes, P.S., Silva, M.A. et al. Growth and phenotypic expression of human endothelial cells cultured on a glass-reinforced hydroxyapatite. J Mater Sci: Mater Med 20, 725–731 (2009). https://doi.org/10.1007/s10856-008-3628-6

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  • DOI: https://doi.org/10.1007/s10856-008-3628-6

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