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Surface silver-doping of biocompatible glass to induce antibacterial properties. Part I: massive glass

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Abstract

A glass belonging to the system SiO2–Al2O3–CaO–Na2O has been subjected to a patented ion-exchange treatment to induce surface antibacterial activity by doping with silver ions. Doped samples have been characterized by means of X-Ray diffraction (XRD), scanning electron microscopy (SEM) observation, energy dispersion spectrometry (EDS) analysis, in vitro bioactivity test, Ag+ leaching test by graphite furnace atomic absorption spectroscopy (GFAAS) analyses, cytotoxicity tests by fibroblasts adhesion and proliferation, adsorption of IgA and IgG on to the material to evaluate its inflammatory property and antibacterial tests (cultures with Staphylococcus aureus and Escherichia coli). In vitro tests results demonstrated that the modified glass maintains the same biocompatibility of the untreated one and, moreover, it acquires an antimicrobial action against tested bacteria. This method can be selected to realize glass or glass-ceramic bone substitutes as well as coatings on bio-inert devices, providing safety against bacterial colonization thus reducing the risks of infections nearby the implant site. The present work is the carrying on of a previous research activity, concerning the application of an ion-exchange treatment on glasses belonging to the ternary system SiO2–CaO–Na2O. On the basis of previous results the glass composition was refined and the ion-exchange process was adapted to it, in order to tune the final material properties. The addition of Al2O3 to the original glass system and the optimization of the ion-exchange conditions allowed a better control of the treatment, leading to an antibacterial material, without affecting both bioactivity and biocompatibility.

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Acknowledgments

The authors would like to acknowledge the financial support of INSTM and Regione Piemonte, that partially funded the research activity.

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Author notes

  1. A. Massé

    Present address: Unit of Orthopaedics and Traumatology, Hospital S. Luigi Gonzaga, University of Torino, Torino, Italy

  2. S. Di Nunzio

    Present address: LINCE Lab. c/o Materials Science and Chemical Engineering Department, Politecnico di Torino, INSTM U.d.R. Politecnico di Torino, Torino, Italy

Authors and Affiliations

  1. Materials Science and Chemical Engineering Department, Polytechnic of Turin, Turin, Italy

    E. Verné, M. Miola, C. Vitale Brovarone & S. Di Nunzio

  2. Department of Medical Sciences, Human Anatomy, University Eastern Piedmont “A. Avogadro”, Novara, Italy

    M. Cannas & S. Gatti

  3. Chemical, Clinical and Microbiological Analyses Department, CTO, Torino, Italy

    G. Fucale

  4. Traumatology Orthopaedics and Occupational Medicine Department, University of Torino, Torino, Italy

    G. Maina & A. Massé

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  1. E. Verné
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Correspondence to E. Verné.

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Verné, E., Miola, M., Vitale Brovarone, C. et al. Surface silver-doping of biocompatible glass to induce antibacterial properties. Part I: massive glass. J Mater Sci: Mater Med 20, 733–740 (2009). https://doi.org/10.1007/s10856-008-3617-9

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  • DOI: https://doi.org/10.1007/s10856-008-3617-9

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