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PLD bioactive ceramic films: the influence of CaO–P2O5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells

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Abstract

This work consists on the evaluation of the in vitro performance of Ti6Al4V samples PLD (pulsed laser deposition) coated with hydroxyapatite, both pure and mixed with a CaO–P2O5 glass. Previous studies on immersion of PLD coatings in SBF, showed that the immersion apatite films did not present the usual cauliflower morphology but replicated the original columnar structure and exhibited good bioactivity. However, the influence of glass associated to hydroxyapatite concerning adhesion, proliferation and morphology of MG63 cells on the films surface was unclear. In this study, the performance of these PLD coated samples was evaluated, not only following the physical–chemical transformations resulting from the SBF immersion, but also evaluating the cytocompatibility in contact with osteoblast-like MG63 cells. SEM and AFM confirmed that the bioactive ceramic PLD films reproduce the substrate’s surface topography and that the films presented good adherence and uniform surface roughness. Physical–chemical phenomena occurring during immersion in SBF did not modify the original columnar structure. In contact with MG63 cells, coated samples exhibited very good acceptance and cytocompatibility when compared to control. The glass mixed with hydroxyapatite induced higher cellular proliferation. Cells grown on these samples presented many filipodia and granular structures, typical features of osteoblasts.

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Authors and Affiliations

  1. Laboratório de Biomateriais, INEB - Instituto de Engenharia Biomédica, Rua do Campo Alegre 823, 4150-180, Porto, Portugal

    Gisela Marta Oliveira, Maria P. Ferraz & Fernando J. Monteiro

  2. Universidade Fernando Pessoa, Praça Nove de Abril 349, 4249-004, Porto, Portugal

    Gisela Marta Oliveira & Maria P. Ferraz

  3. Departamento de Física Aplicada, Universidad de Vigo, Lagoas, Marcosende, 9, 36280, Vigo, Spain

    Pío G. González, Julia Serra, Betty Leon & Mariano Pèrez-Amor

  4. Departamento de Engenharia Metalúrgica e de Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal

    Fernando J. Monteiro

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  1. Gisela Marta Oliveira
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  2. Maria P. Ferraz
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Correspondence to Gisela Marta Oliveira.

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Oliveira, G.M., Ferraz, M.P., González, P.G. et al. PLD bioactive ceramic films: the influence of CaO–P2O5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells. J Mater Sci: Mater Med 19, 1775–1785 (2008). https://doi.org/10.1007/s10856-007-3331-z

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  • DOI: https://doi.org/10.1007/s10856-007-3331-z

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