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The effect of adsorbed vitamin D and K to hydroxyapatite on ALP activity of MC3T3-E1 cell

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Abstract

This study describes the adsorptive property of vitamins on HA and the effect of the HA adsorbed vitamin on the alkaline phosphatase (ALP) activity for effective use as a bone graft substitute. The vitamins used were calciferol (D3), menaquirone (K2) and 25-hydroxycholecalciferol (25(OH)D3). These vitamins were adsorbed on HA at 4, 10, 20, 37 and 50 °C. The adsorption amount was constant below 20 °C, and decreased as the incubation temperature increased over 20 °C. The order of the adsorption amount was: 25(OH)D3 > K2 > D3. The HA adsorbed vitamins (HA/D3, HA/25(OH)D3 and HA/K2) were suspended in physiological saline for 48 h for the release test. The release ratio of all vitamins increased with incubation time. The order of the release ratio was: 25(OH)D3 > K2 > D3, which was proportional to that of the adsorption amount. The ALP activity of MC3T3-E1 osteoblast-like cells on HA, HA/D3, HA/25(OH)D3 and HA/K2 was also investigated. The ALP activity was higher on HA/25(OH)D3 than on any other samples. However, HA/K2 and HA/D3 showed similar ALP activity to HA.

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Authors and Affiliations

  1. Department of Mechanical Engineering, Ibaraki University, 4-12-1, Nakanarusawa, Hitachi, Ibaraki, 316-8511, Japan

    K. Ozeki

  2. International Apatite Co., Ltd, 20 Kanda-Ogawamachi 3-Chome, Chiyoda-ku, Tokyo, 101-0052, Japan

    H. Aoki

  3. Applied Systems Engineering, Graduate School of Science and Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Hiki, Saitama, 350-0394, Japan

    Y. Fukui

Authors
  1. K. Ozeki
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  2. H. Aoki
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  3. Y. Fukui
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Correspondence to K. Ozeki.

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Ozeki, K., Aoki, H. & Fukui, Y. The effect of adsorbed vitamin D and K to hydroxyapatite on ALP activity of MC3T3-E1 cell. J Mater Sci: Mater Med 19, 1753–1757 (2008). https://doi.org/10.1007/s10856-007-3288-y

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  • DOI: https://doi.org/10.1007/s10856-007-3288-y

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